PD ISO/TS 19006:2016
Current
The latest, up-to-date edition.
Nanotechnologies. 5-(and 6)-Chloromethyl-2’,7’ Dichlorodihydrofluorescein diacetate (CM-H2DCF- DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line
Hardcopy , PDF
English
31-08-2016
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Symbols and abbreviated terms
5 Materials
6 Technical equipment
7 Nanoparticle sample preparation
8 Preparations
9 Evaluation of nanoparticle impact on ROS generation in cells
10 Data analysis and results
Annex A (informative) - Alternate cell lines
Annex B (informative) - Alternate fluorescence characterization
techniques
Annex C (informative) - Suspension preparation and characterization
Annex D (informative) - Example experimental data from RAW 264.7
Bibliography
Defines how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H[2]DCFDA assay.
| Committee |
NTI/1
|
| DocumentType |
Standard
|
| Pages |
32
|
| PublisherName |
British Standards Institution
|
| Status |
Current
|
This document describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H2DCFDA assay.
The protocol in this document is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.
| Standards | Relationship |
| ISO/TS 19006:2016 | Identical |
| ISO 29701:2010 | Nanotechnologies Endotoxin test on nanomaterial samples for in vitro systems Limulus amebocyte lysate (LAL) test |
| ISO/TS 18827:2017 | Nanotechnologies — Electron spin resonance (ESR) as a method for measuring reactive oxygen species (ROS) generated by metal oxide nanomaterials |
| ISO/TR 13097:2013 | Guidelines for the characterization of dispersion stability |
| ISO/TS 80004-2:2015 | Nanotechnologies — Vocabulary — Part 2: Nano-objects |
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