PREN 13623 : DRAFT 2008

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Requirements
5 Test methods
  5.1 Principle
  5.2 Materials and reagents
       5.2.1 Test organism
       5.2.2 Culture media and reagents
  5.3 Apparatus and glassware
       5.3.1 General
       5.3.2 Usual microbiological laboratory equipment
  5.4 Preparation of test organism suspensions and test
       solutions
       5.4.1 Test organism suspension (test and validation
             suspension)
       5.4.2 Product test solution
  5.5 Procedure for assessing the bactericidal activity of
       the product
       5.5.1 General
       5.5.2 Dilution-neutralization method
       5.5.3 Membrane filtration method
  5.6 Experimental data and calculations
       5.6.1 Explanation of terms and abbreviations
       5.6.2 Calculation
  5.7 Verification of methodology
       5.7.1 General
       5.7.2 Control of weighted mean counts
       5.7.3 Basic limits
  5.8 Expression of results and precision
       5.8.1 Reduction
       5.8.2 Control of active and non-active product test
             solution (5.4.2)
       5.8.3 Bactericidal concentration
       5.8.4 Precision, repetition
  5.9 Interpretation of results - conclusion
       5.9.1 General
       5.9.2 Bactericidal activity for general purposes
       5.9.3 Bactericidal activity for specific purposes
  5.10 Test report
Annex A (informative) Determination of the bactericidal
        activity against Legionella pneumophila
Annex B (informative) Neutralizer
Annex C (informative) Graphical representation of test procedures
      C.1 Dilution-neutralization method
      C.2 Membrane filtration method
Bibliography

Defines a test method and the minimum requirements for bactericidal activity of chemical disinfectant products intended to be used for treatment in aqueous systems against Legionella pneumophila that form a homogeneous, physically stable preparation when diluted with buffered ferrous hard water or hard water.