PREN 16418 : DRAFT 2012
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
View Superseded by
PAPER AND BOARD - DETERMINATION OF THE CYTOTOXICITY OF AQUEOUS EXTRACTS USING A METABOLICALLY COMPETENT HEPATOMA CELL LINE (HEPG2)
01-04-2014
12-01-2013
Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Cell line
7 Food simulants used for testing
8 Cleaning laboratory glassware
9 Equipment
10 Preparation of specimens
11 Cytotoxicity assessment
12 Expression of the results
13 Interpretation of the results
14 Precision
15 Test report
Annex A (informative) - 96-well plates configuration
Annex B (informative) - RNA Synthesis rate inhibition
cytotoxicity test work flow
Annex C (informative) - Validation of the two methods
(option A and B)
Bibliography
Defines a test method for the laboratory assessment of the potential cytotoxic effect of paper and board food contact materials using specifically the HepG2 cell line.
| Committee |
TC 172
|
| DocumentType |
Draft
|
| PublisherName |
Comite Europeen de Normalisation
|
| Status |
Superseded
|
| SupersededBy |
| Standards | Relationship |
| 12/30259424 DC : 0 | Identical |
| NEN EN 16418 : 2014 | Identical |
| EN 645:1993 | Paper and board intended to come into contact with foodstuffs - Preparation of a cold water extract |
| EN 15845:2010 | Paper and board - Determination of the cytotoxicity of aqueous extracts |
| ISO 3696:1987 | Water for analytical laboratory use — Specification and test methods |
| EN ISO 3696:1995 | Water for analytical laboratory use - Specification and test methods (ISO 3696:1987) |
| EN 647:1993 | Paper and board intended to come into contact with foodstuffs - Preparation of a hot water extract |
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