UNE-EN ISO 12966-2:2017
Current
The latest, up-to-date edition.
Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 2: Preparation of methyl esters of fatty acids (ISO 12966-2:2017)
Hardcopy , PDF
Spanish, Castilian, English
12-07-2017
This document specifies methods of preparing the methyl esters of fatty acids.
It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty
acids and soaps. To cover different requirements, four methylation methods are specified, namely:
a) a rapid transmethylation procedure under alkaline conditions;
b) a general transmethylation/methylation procedure under sequential alkaline and acid conditions;
c) a BF3 transmethylation procedure;
d) an alternative procedure using acid-catalysed transmethylation of glycerides.
Methyl esters so produced are used in various analytical procedures requiring such derivatives, e.g.
gas-liquid chromatography (GLC), thin-layer chromatography (TLC) and infrared spectrometry (IR).
Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this
document.
| Committee |
CTN 34/SC 6
|
| DevelopmentNote |
Supersedes UNE EN ISO 5509. (07/2011)
|
| DocumentType |
Standard
|
| Pages |
27
|
| PublisherName |
Asociación Española de Normalización
|
| Status |
Current
|
| Standards | Relationship |
| EN ISO 12966-2:2017 | Identical |
| ISO 12966-2:2017 | Identical |
| ISO 1042:1998 | Laboratory glassware — One-mark volumetric flasks |
| ISO 835:2007 | Laboratory glassware — Graduated pipettes |
| ISO 4799:1978 | Laboratory glassware — Condensers |
| ISO 8655-2:2002 | Piston-operated volumetric apparatus — Part 2: Piston pipettes |
| ISO 661:2003 | Animal and vegetable fats and oils — Preparation of test sample |
| EN ISO 3696:1995 | Water for analytical laboratory use - Specification and test methods (ISO 3696:1987) |
| ISO 78-2:1999 | Chemistry — Layouts for standards — Part 2: Methods of chemical analysis |
| ISO 4800:1998 | Laboratory glassware — Separating funnels and dropping funnels |
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