UNE-EN ISO 15216-2:2020
Current
The latest, up-to-date edition.
Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 2: Method for detection (ISO 15216-2:2019)
Hardcopy , PDF
English, Spanish, Castilian
01-07-2020
This document specifies a method for detection of HAV and norovirus genogroups I (GI) and II (GII), from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.This method is not validated for detection of the target viruses in other foodstuffs (including multicomponent foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.
| Committee |
CTN 34/SC 4
|
| DocumentType |
Standard
|
| Pages |
50
|
| PublisherName |
Asociación Española de Normalización
|
| Status |
Current
|
| Standards | Relationship |
| ISO 15216-2:2019 | Identical |
| EN ISO 15216-2:2019 | Identical |
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