UNE-EN ISO 16387:2023

This document specifies one of the methods for evaluating the habitat function of soils and determiningeffects of soil contaminants and substances on the reproduction of Enchytraeus sp. by dermal andalimentary uptake in a chronic test. It is applicable to soils and soil materials of unknown quality,e.g. from contaminated sites, amended soils, soils after remediation, agricultural or other sites underconcern and waste materials.Effects of substances are assessed using a standard soil, preferably a defined artificial soil substrate.For contaminated soils, the effects are determined in the soil to be tested and in a control soil. Accordingto the objective of the study, the control and dilution substrate (dilution series of contaminated soil) areeither an uncontaminated soil comparable to the soil to be tested (reference soil) or a standard soil (e.g.artificial soil).This document provides information on how to use this method for testing substances under temperateconditions.The method is not applicable to substances, for which the air/soil partition coefficient is greater than 1,or to substances for which the vapour pressure exceeds 300 Pa at 25 °C.NOTE No provision is made in the test method for monitoring the persistence of the substance under test.