UNI EN 12821 : 2009
Current
The latest, up-to-date edition.
FOODSTUFFS - DETERMINATION OF VITAMIN D BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY - MEASUREMENT OF CHOLECALCIFEROL (D3) OR ERGOCALCIFEROL (D2)
18-06-2009
Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Procedure
7 Calculation
8 Precision
9 Test report
Annex A (informative) - Examples of suitable HPLC
systems
Annex B (informative) - Examples of suitable extraction
and saponification conditions
Annex C (normative) - Examples of suitable semi-preparative
and analytical HPLC chromatograms
Annex D (informative) - Precision data
Annex E (informative) - Additional cleanup step for the
determination of vitamin D with use of preparative
TLC, column chromatography and or SPE
Bibliography
Describes a method for the determination of vitamin D[3] (cholecalciferol) or vitamin D[2] (ergocalciferol) in foodstuffs by high performance liquid chromatography (HPLC).
| Committee |
CT 3
|
| DocumentType |
Standard
|
| PublisherName |
Ente Nazionale Italiano di Unificazione (UNI)
|
| Status |
Current
|
| Standards | Relationship |
| DIN EN 12821:2009-08 | Identical |
| I.S. EN 12821:2009 | Identical |
| NEN EN 12821 : 2009 | Identical |
| UNE-EN 12821:2009 | Identical |
| EN 12821:2009 | Identical |
| BS EN 12821:2009 | Identical |
| NF EN 12821 : 2009 | Identical |
| NBN EN 12821 : 2009 | Identical |
| NS EN 12821 : 2009 | Identical |
| SN EN 12821 : 2009 | Identical |
| ISO 5725:1986 | Precision of test methods Determination of repeatability and reproducibility for a standard test method by inter-laboratory tests |
| ISO 3696:1987 | Water for analytical laboratory use — Specification and test methods |
| EN ISO 3696:1995 | Water for analytical laboratory use - Specification and test methods (ISO 3696:1987) |
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