UNI EN ISO 21871 : 2006

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
   4.1 Enumeration method
   4.2 Detection method
5 Diluent, culture media and reagents
   5.1 General
   5.2 Diluent
   5.3 Liquid selective enrichment medium:
        Tryptone soya polymyxin broth (TSPB)
   5.4 Solid selective medium: Polymyxin
        pyruvate egg yolk mannitol bromothymol
        blue agar (PEMBA)
   5.5 Solid selective medium: Mannitol egg
        yolk polymyxin agar (MYP)
   5.6 Staining solutions for microscopic
        identification
   5.7 Sheep blood agar
6 Apparatus and glassware
7 Sampling
8 Preparation of test sample
9 Procedure
   9.1 Enumeration method
   9.2 Detection method
10 Calculation and expression of results
   10.1 Enumeration method for the determination
        of the most probable number (MPN)
   10.2 Detection method
11 Test report
Annex A (normative) Diagram of enumeration procedure
Bibliography

Describes a horizontal method for the detection or the enumeration of low numbers of viable presumptive Bacillus cereus by means of the most probable number technique.