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UNI EN ISO 21871 : 2006

Current

Current

The latest, up-to-date edition.

MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE DETERMINATION OF LOW NUMBERS OF PRESUMPTIVE BACILLUS CEREUS - MOST PROBABLE NUMBER TECHNIQUE AND DETECTION METHOD

Published date

06-04-2006

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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
   4.1 Enumeration method
   4.2 Detection method
5 Diluent, culture media and reagents
   5.1 General
   5.2 Diluent
   5.3 Liquid selective enrichment medium:
        Tryptone soya polymyxin broth (TSPB)
   5.4 Solid selective medium: Polymyxin
        pyruvate egg yolk mannitol bromothymol
        blue agar (PEMBA)
   5.5 Solid selective medium: Mannitol egg
        yolk polymyxin agar (MYP)
   5.6 Staining solutions for microscopic
        identification
   5.7 Sheep blood agar
6 Apparatus and glassware
7 Sampling
8 Preparation of test sample
9 Procedure
   9.1 Enumeration method
   9.2 Detection method
10 Calculation and expression of results
   10.1 Enumeration method for the determination
        of the most probable number (MPN)
   10.2 Detection method
11 Test report
Annex A (normative) Diagram of enumeration procedure
Bibliography

Describes a horizontal method for the detection or the enumeration of low numbers of viable presumptive Bacillus cereus by means of the most probable number technique.

Committee
CT 3
DocumentType
Standard
PublisherName
Ente Nazionale Italiano di Unificazione (UNI)
Status
Current

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