UNI EN ISO 22174 : 2005
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
View Superseded by
MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - GENERAL REQUIREMENTS AND DEFINITIONS
23-10-2024
01-08-2005
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 General
4.2 Preliminary microbial enrichment
4.3 Nucleic acid preparation
4.4 PCR amplification
4.5 Detection and confirmation of PCR products
5 Test material
6 General laboratory requirements
6.1 General
6.2 Personnel
6.3 Laboratory setup
6.4 Waste management
7 Reagents
8 Apparatus and equipment
8.1 General
8.2 Special considerations
9 Procedure
9.1 Sample preparation
9.2 Amplification
9.3 Control reaction
9.4 Confirmation of PCR results
10 Evaluation
11 Test report
Bibliography
Describes the general requirements for the in vitro amplification of nucleic acid sequences (DNA or RNA). Applicable to the testing of foodstuffs and isolates obtained from foodstuffs for food-borne pathogens using the polymerase chain reaction (PCR).
| Committee |
CT 3
|
| DocumentType |
Standard
|
| PublisherName |
Ente Nazionale Italiano di Unificazione (UNI)
|
| Status |
Superseded
|
| SupersededBy |
| Standards | Relationship |
| NF EN ISO 22174 : 2005 | Identical |
| ISO 22174:2005 | Identical |
| NBN EN ISO 22174 : 2005 | Identical |
| NEN EN ISO 22174 : 2005 | Identical |
| NS EN ISO 22174 : 1ED 2005 | Identical |
| I.S. EN ISO 22174:2005 | Identical |
| UNE-EN ISO 22174:2005 | Identical |
| BS EN ISO 22174:2005 | Identical |
| EN ISO 22174:2005 | Identical |
| DIN EN ISO 22174:2005-05 | Identical |
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