UNI EN ISO 22959 : 2009

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents, materials and standards
6 Apparatus
7 Sampling and preparation of the test sample
8 Sample preparation
9 Procedure
   9.1 HPLC analysis
   9.2 Identification of PAHs
10 Calculation of individual PAHs
11 Method validation data
   11.1 Accuracy
   11.2 Within-laboratory precision
   11.3 Recovery
   11.4 Dynamic range
   11.5 Limit of quantification
12 Precision
   12.1 International collaborative trial
   12.2 Repeatability
   12.3 Reproducibility
13 Test report
Annex A (informative) - Example of the individual parts of
        an HPLC system
Annex B (informative) - Example of the operating conditions
        of the pumps of the HPLC system
Annex C (informative) - Example of the operating conditions
        of the column thermostat and the detector of the HPLC
        system
Annex D (informative) - Example of the autosampler
        programme of the HPLC system
Annex E (informative) - Tubing connections of the HPLC
        system
Annex F (informative) - Chromatogram of a standard
        calibration sample
Annex G (informative) - Determination precision for a
        sunflower oil, range 0,1 [mu]g/kg to 3,5 [mu]g/kg
Annex H (informative) - Determination precision for an olive
        oil, range 0,1 [mu]g/kg to 3,5 [mu]g/kg
Annex I (informative) - Determination precision for a soybean
        oil, range 0,1 [mu]g/kg to 3,5 [mu]g/kg
Annex J (informative) - Determination precision for a coconut
        oil, range 0,1 [mu]g/kg to 3,5 [mu]g/kg
Annex K (informative) - Interlaboratory test
Bibliography

Describes a high performance liquid chromatographic (HPLC) procedure for the determination of polycyclic aromatic hydrocarbons (PAHs) in edible fats and oils.