Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Test environment
6 Reagents, test organisms and media
7 Apparatus and materials
8 Treatment and preparation of samples
9 Procedure
10 Interpretation and validity of the results
11 Expression of results
12 Test report
Annex A (informative) - Preparation of the Elendt M4 medium
Annex B (informative) - Example of graphical determination of
the inhibition of mobility of Daphnia magna by an
effluent or stock solution of a substance at a
concentration of 1000 mg/l
Annex C (informative) - General recommendations for stock
culturing
Annex D (informative) - Culturing of Daphnia magna for
production of dormant eggs
Annex E (informative) - Precision data
Annex F (informative) - Dilution level D - Preparation of
the dilution series for the determination of the LID
Bibliography