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    AS 2252.2-2004

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    Superseded A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
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    Biological safety cabinets - Laminar flow biological safety cabinets (Class II) for personnel, environment and product protection

    Available format(s):  Hardcopy, PDF 1 User, PDF 3 Users, PDF 5 Users, PDF 9 Users

    Superseded date:  26-06-2021

    Language(s):  English

    Published date:  01-01-2004

    Publisher:  Standards Australia

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    Table of Contents - (Show below) - (Hide below)

    1 - AS 2252.2-2004 BIOLOGICAL SAFETY CABINETS - LAMINAR FLOW BIOLOGICAL SAFETY CABINETS (CLASS II) FOR PERSONNEL...
    4 - PREFACE
    6 - CONTENTS
    7 - FOREWORD
    9 - 1 SCOPE
    9 - 2 REFERENCED DOCUMENTS
    10 - 3 GENERAL REQUIREMENTS
    14 - 4 CONSTRUCTION REQUIREMENTS
    14 - 4.1 General
    14 - 4.2 Outer shell
    14 - 4.3 Work zone
    14 - 4.3.1 General
    14 - 4.3.2 Viewing windows
    14 - 4.3.3 Work access opening
    15 - 4.3.4 Work access opening cover
    15 - 4.3.5 Work zone illumination
    15 - 4.3.6 Work floor
    15 - 4.3.7 Grilles
    15 - 4.3.8 Sump
    15 - 4.4 Filters and filter installations
    16 - 4.5 Blowers and alarm systems
    16 - 4.5.1 Blowers
    16 - 4.5.2 Blower alarm systems
    16 - 4.6 Access panels
    17 - 4.7 Electrical services
    17 - 4.8 Germicidal ultraviolet (UV) lamps
    17 - 4.9 Gas supply
    18 - 4.10 Adsorbent filter
    18 - 4.11 Post-use overrun control
    18 - 4.12 Decontamination covers
    18 - 5 PERFORMANCE REQUIREMENTS
    18 - 5.1 General
    18 - 5.2 Critical for cabinet function
    18 - 5.2.1 Filter installation integrity
    18 - 5.2.2 Air barrier containment
    18 - 5.2.3 Work zone integrity
    19 - 5.2.4 Air velocity and uniformity in the work zone
    19 - 5.2.5 Alarm operational adjustment
    19 - 5.3 Vibration
    19 - 5.4 Other requirements
    19 - 5.4.1 Sound level
    19 - 5.4.2 Lighting
    19 - 5.4.3 Ultraviolet radiation
    20 - 6 MARKING
    21 - APPENDIX A - ERGONOMICS
    21 - A1 GENERAL
    21 - A2 CONSIDERATIONS OF ERGONOMICS FOR SAFETY CABINETS AND ISOLATORS
    21 - A2.1 General
    22 - A2.2 Design
    23 - A2.3 Purchase and use

    Abstract - (Show below) - (Hide below)

    Specifies general construction and performance requirements for Class II laminar flow biological safety cabinets intended to provide protection for hazardous biological agents for personnel and the environment and also to protect material used in the cabinet from exogenous contamination.

    Scope - (Show below) - (Hide below)

    This Standard specifies basic requirements for Class II laminar flow biological safety cabinets that are intended to provide protection from hazardous biological agents for personnel and the environment and also to protect material used in the cabinet from exogenous contamination. The cabinets provide protection by inducing an inflow of room air through the work access opening, by delivering recirculated, high efficiency particulate air (HEPA) filtered, laminar flow air downwards through the work zone and by HEPA filtration of exhaust air. It is essential that this Standard be read in conjunction with AS/NZS 2647 which describes recommended practices for installation and use of these cabinets. NOTES: 1 These cabinets are intended only for handling materials which can be inactivated or rendered safe by an effective decontamination procedure such as that described in AS/NZS 2647. 2 Additional design requirements may apply to cabinets which are required to afford protection against other hazards such as toxic materials not of biological origin or against radiation.3 For work with cytotoxic drugs the user is referred to AS 2567.4 Ergonomic considerations for the design and selection of biological safety cabinets are addressed in Appendix A.

    General Product Information - (Show below) - (Hide below)

    Committee ME-060
    Document Type Standard
    Publisher Standards Australia
    Status Superseded
    Superseded By
    Supersedes
    Under Revision

    History - (Show below) - (Hide below)

    First published as AS 2252.2-1980.Second edition 1985.Third edition 1994.Fourth edition 2004. First published as AS 2252.2-1980. Second edition 1985. Third edition 1994. Fourth edition 2004.

    Standards Referenced By This Book - (Show below) - (Hide below)

    AS 1807.23-2000 Cleanrooms, workstations, safety cabinets and pharmaceutical isolators - Methods of test Determination of intensity of radiation from germicidal ultraviolet lamps
    AS 1807.26-2004 Cleanrooms, workstations, safety cabinets and pharmaceutical isolators - Methods of test Determination of air barrier containment of laminar flow safety cabinets - Potassium iodidediscus test
    AS/NZS 2647:2000 Biological safety cabinets - Installation and use
    AS 1807.2-2000 Cleanrooms, workstations, safety cabinets and pharmaceutical isolators - Methods of test Determination of performance of clean workstations, laminar flow safety cabinets and pharmaceutical isolators under loaded filter conditions
    AS 1807.22-2000 Cleanrooms, workstations, safety cabinets and pharmaceutical isolators - Methods of test Determination of air barrier containment of laminar flow safety cabinets
    AS/NZS 2243.3:2002 Safety in laboratories Microbiological aspects and containment facilities
    AS 1319-1994 Safety signs for the occupational environment (Reconfirmed 2018)
    AS/NZS 3000:2000 Electrical installations (known as the Australian/New Zealand Wiring Rules)
    AS 4260-1997 High efficiency particulate air (HEPA) filters - Classification, construction and performance (Reconfirmed 2018)
    AS 1807.15-2000 Cleanrooms, workstations, safety cabinets and pharmaceutical isolators - Methods of test Determination of illuminance
    AS 1807.6-2000 Cleanrooms, workstations, safety cabinets and pharmaceutical isolators - Methods of test Determination of integrity of terminally mounted HEPA filter installations
    AS/NZS 2107:2000 Acoustics - Recommended design sound levels and reverberation times for building interiors
    AS/NZS 3100:2002 Approval and test specification - General requirements for electrical equipment
    AS 2567-2002 Laminar flow cytotoxic drug safety cabinets
    AS 1939-1990 Degrees of protection provided by enclosures for electrical equipment (IP Code)
    AS 2659.1-1988 Guide to the use of sound measuring equipment - Portable sound level meters
    AS 1807.5-2000 Cleanrooms, workstations, safety cabinets and pharmaceutical isolators - Methods of test Determination of work zone integrity
    AS 1807.1-2000 Cleanrooms, workstations, safety cabinets and pharmaceutical isolators - Methods of test Determination of air velocity and uniformity of air velocity in clean workstations, laminar flow safety cabinets and pharmaceutical isolators
    AS 1807.18-2000 Cleanrooms, workstations, safety cabinets and pharmaceutical isolators - Methods of test Determination of vibration in workstations, safety cabinets and pharmaceutical isolators
    AS 2252.1-2002 Biological safety cabinets - Biological safety cabinets (Class I) for personnel and environment protection

    Standards Referencing This Book - (Show below) - (Hide below)

    AS/NZS 2243.3:2002 Safety in laboratories Microbiological aspects and containment facilities
    AS 1386.1-1989 Cleanrooms and clean workstations - Principles of clean space control
    AS 3870-1991 Certified reference materials - General guide to material selection, preparation, testing and certification
    AS/NZS 2869:1998 Tampons - Menstrual
    AS 2411-1993 Venous blood - Determination of lead content - Flame atomic absorption spectrometric method
    AS 2830.1-1985 Good laboratory practice - Chemical analysis
    AS/NZS 2647:2000 Biological safety cabinets - Installation and use
    AS/NZS 2243.1:2005 Safety in laboratories Planning and operational aspects
    AS 1386.5-1989 Cleanrooms and clean workstations - Clean workstations
    AS/NZS 2243.9:2009 Safety in laboratories Recirculating fume cabinets
    AS 2869-2008 Tampons - Menstrual
    AS 1807.22-2000 Cleanrooms, workstations, safety cabinets and pharmaceutical isolators - Methods of test Determination of air barrier containment of laminar flow safety cabinets
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