AS/NZS 4308:2001

1 - AS/NZS 4308:2001 PROCEDURES FOR THE COLLECTION, DETECTION AND QUANTITATION OF DRUGS OF ABUSE IN URINE
4 - PREFACE
5 - CONTENTS
7 - SECTION 1 SCOPE AND GENERAL
7 - 1.1 SCOPE
7 - 1.2 REFERENCED DOCUMENTS
8 - 1.3 DEFINITIONS
8 - 1.3.1 Benzodiazepines
8 - 1.3.2 Calibration standard
8 - 1.3.3 Cannabis metabolite
8 - 1.3.4 Chain-of-custody
8 - 1.3.5 Chain-of-custody form
8 - 1.3.6 Cocaine metabolites
8 - 1.3.7 Collection site
8 - 1.3.8 Collector
8 - 1.3.9 Confirmatory test
8 - 1.3.10 Control sample
8 - 1.3.11 Cut-off level
8 - 1.3.12 Drug-free
8 - 1.3.13 Initial test
9 - 1.3.14 Laboratory
9 - 1.3.15 Laboratory sample
9 - 1.3.16 Opiates
9 - 1.3.17 Permanent record system
9 - 1.3.18 Proficiency testing program
9 - 1.3.19 Referee sample
9 - 1.3.20 Reference compound
9 - 1.3.21 Sympathomimetic amines
9 - 1.3.22 Test portion
9 - 1.4 PRINCIPLE
9 - 1.5 REAGENTS
10 - 1.6 APPARATUS
10 - 1.6.1 Volumetric glassware
10 - 1.6.2 Piston operated volumetric apparatus
10 - 1.6.3 Thermometer
10 - 1.6.4 Clinical chemistry analyser
10 - 1.6.5 Gas chromatograph
10 - 1.6.6 Gas chromatograph/mass spectrometer
10 - 1.6.7 High pressure liquid chromatograph
10 - 1.6.8 Liquid chromatograph/mass spectrometer
11 - SECTION 2 PERSONNEL
11 - 2.1 DAY-TO-DAY MANAGEMENT
11 - 2.2 TEST VALIDATION
11 - 2.3 OPERATION AND SUPERVISION OF ANALYSTS
12 - SECTION 3 SPECIMEN COLLECTION AND STORAGE PROCEDURES
12 - 3.1 GENERAL
12 - 3.2 COLLECTION SITE
12 - 3.2.1 General
12 - 3.2.2 Privacy
12 - 3.2.3 Security
12 - 3.2.4 Chain-of-custody
12 - 3.2.5 Access
12 - 3.3 INTEGRITY AND IDENTITY OF THE COLLECTED SPECIMEN
12 - 3.3.1 General
13 - 3.3.2 Precautions
13 - 3.3.3 Collection procedure
14 - 3.3.4 Preparation for dispatch
14 - 3.4 TRANSPORTATION TO THE LABORATORY
14 - 3.5 SPECIMEN RECEPTION
15 - 3.6 LABORATORY SECURITY
15 - 3.7 STORAGE OF LABORATORY SAMPLES
15 - 3.7.1 Short-term refrigerated storage
15 - 3.7.2 Long-term refrigerated storage
16 - SECTION 4 INITIAL TESTING
16 - 4.1 GENERAL
16 - 4.2 METHOD
16 - 4.3 NUMBER OF DETERMINATIONS
17 - 4.4 BLANK DETERMINATION
17 - 4.5 QUALITY CONTROL
17 - 4.6 INITIAL TEST CUT-OFF LEVELS
17 - 4.7 ACCEPTANCE OF RESULTS
17 - 4.8 INTERPRETATION OF RESULTS
19 - SECTION 5 CONFIRMATORY TESTING PROCEDURES
19 - 5.1 CONFIRMATION OF INITIAL TESTING
19 - 5.2 INSTRUMENT CONDITIONS
19 - 5.3 NUMBER OF DETERMINATIONS
19 - 5.4 BLANK DETERMINATION
19 - 5.5 INSTRUMENT SETUP
19 - 5.6 QUALITY CONTROL
20 - 5.7 CONFIRMATORY TEST CUT-OFF LEVELS
20 - 5.8 CALCULATIONS
20 - 5.9 ACCEPTANCE OF RESULTS
22 - SECTION 6 REPORTING OF RESULTS
22 - 6.1 TEST REPORT
22 - 6.2 RECORD KEEPING
22 - 6.3 DISPUTED RESULTS
23 - APPENDIX A - EXAMPLE OF CHAIN-OF-CUSTODY FORM
25 - APPENDIX B - RECOMMENDED PRECAUTIONS FOR HANDLING BIOLOGICAL SPECIMENS
25 - B1 GENERAL RECOMMENDATIONS
25 - B2 DISINFECTION OF SPILLS
26 - B3 DISINFECTION OF EQUIPMENT
26 - B4 WASTE DISPOSAL
26 - B5 BIBLIOGRAPHY
27 - APPENDIX C - PRINCIPLES OF OPERATION
27 - C1 GAS CHROMATOGRAPHY
27 - C2 GAS CHROMATOGRAPHY/MASS SPECTROMETRY
27 - C3 HIGH PRESSURE LIQUID CHROMATOGRAPHY
27 - C4 IMMUNOASSAY
28 - C5 LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY

Sets out the procedures for the sample collection and detection and quantitation of drugs of abuse in human urine. The method may be used for workplace, medicolegal or court directed detection of any or all of the following classes of drugs: opiates, simpathoamimetic amines, cannabis metabolites, cocaine metabolites or benzodiazepines.

This Standard sets out procedures for the sample collection and the detection and quantitation of drugs of abuse in human urine. The method is intended for workplace, medicolegal or court directed detection of any or all of the following classes of drugs:(a) Opiates.(b) Sympathomimetic amines.(c) Cannabis metabolites.(d) Cocaine metabolites.(e) Benzodiazepines.NOTE: The detection and reporting of drug classes other than those listed above is not precluded. See Clause 6.1(g).Aspects of this Standard have relevance to testing for drugs of abuse in urine; however, clinical testing is outside the scope of this Standard. This Standard does not cover the testing of urine samples following excessive intake of therapeutic drugs or vitamins, exogenous hormone intake or ingestion of performance-enhancing drugs which may be used by sports participants.Procedures for initial testing are set out in Section 4. Procedures for confirmatory testing are set out in Section 5.NOTE: It is a requirement of this Standard that initial testing and confirmatory testing are performed in the same laboratory (see Clauses 4.1 and 5.1.

First published as AS 4308-1995.
Jointly revised and designated as AS/NZS 4308:2001.