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ASTM D 3338/D3338M : 2009 : R2014 : EDT 2

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

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Standard Test Method for Estimation of Net Heat of Combustion of Aviation Fuels

Available format(s)

Hardcopy , PDF

Superseded date

24-06-2020

Language(s)

English

Published date

01-05-2014

€59.22
Excluding VAT

CONTAINED IN VOL. 05.02, 2015 Describes the estimation of the net heat of combustion (megajoules per kilogram or [Btu per pound]) of aviation gasolines and aircraft turbine and jet engine fuels in the range from 40.19 to 44.73 megajoules per kilogram or [17280 to 19230 Btu per pound].

Committee
D 02
DevelopmentNote
Supersedes ASTM D 3338. (05/2009)
DocumentType
Test Method
Pages
4
ProductNote
Reconfirmed EDT 2
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy
Supersedes

1.1This test method covers the estimation of the net heat of combustion (megajoules per kilogram or [Btu per pound]) of aviation gasolines and aircraft turbine and jet engine fuels in the range from 40.19 to 44.73 megajoules per kilogram or [17 280 to 19 230 Btu per pound]. The precision for estimation of the net heat of combustion outside this range has not been determined for this test method.

1.2This test method is purely empirical and is applicable to liquid hydrocarbon fuels that conform to the specifications for aviation gasolines or aircraft turbine and jet engine fuels of grades Jet A, Jet A-1, Jet B, JP-4, JP-5, JP-7, and JP-8.

Note 1:The experimental data on heat of combustion from which the Test Method D3338 correlation was devised was obtained by a precision method similar to Test Method D4809.

Note 2:The estimation of the net heat of combustion of a hydrocarbon fuel is justifiable only when the fuel belongs to a well-defined class for which a relation between heat of combustion and aromatic and sulfur contents, density, and distillation range of the fuel has been derived from accurate experimental measurements on representative samples of that class. Even in this case, the possibility that the estimates may be in error by large amounts for individual fuels should be recognized. The fuels used to establish the correlation presented in this method are defined as follows:

Fuels:

Aviation gasoline—Grades 100/130 and 115/145 (1, 2)2

Kerosines, alkylates, and special WADC fuels (3)

Pure hydrocarbons—paraffins, naphthenes, and aromatics (4)

Fuels for which data were reported by the Coordinating Research Council (5).

Note 3:The property ranges used in this correlation are as follows:

Aromatics—from 0 mass percent to 100 mass percent

API Gravity—from [25.7 ° to 81.2 °API]

Volatility—from [160 °F to 540 °F], average boiling point

1.3The values stated in either SI units or inch-pound units are to be regarded separately as standard. The values stated in each system may not be exact equivalents; therefore, each system shall be used independently of the other. Combining values from the two systems may result in non-conformance with the standard.

1.3.1Although the test method permits the calculation of net heat of combustion in either SI or inch-pound units, SI units are the preferred units.

1.3.2The net heat of combustion can also be estimated in inch-pound units by Test Method D1405 or in SI units by Test Method D4529. Test Method D1405 requires calculation of one of four equations dependent on the fuel type with a precision equivalent to that of this test method. Test Method D4529 requires calculation of a single equation for all aviation fuels with a precision equivalent to that of this test method. Unlike Test Method D1405 and D4529, Test Method D3338/D3338M does not require the use of aniline point.

1.4This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

ASTM D 2622 : 1992 : EDT 1 Standard Test Method for Sulfur in Petroleum Products by X-Ray Spectrometry
ASTM D 5453 : 2009 Standard Test Method for Determination of Total Sulfur in Light Hydrocarbons, Spark Ignition Engine Fuel, Diesel Engine Fuel, and Engine Oil by Ultraviolet Fluorescence
ASTM D 1552 : 2003 Standard Test Method for Sulfur in Petroleum Products (High-Temperature Method)
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ASTM D 4529 : 2017 Standard Test Method for Estimation of Net Heat of Combustion of Aviation Fuels
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ASTM D 5453 : 2008 : REV B Standard Test Method for Determination of Total Sulfur in Light Hydrocarbons, Spark Ignition Engine Fuel, Diesel Engine Fuel, and Engine Oil by Ultraviolet Fluorescence
ASTM D 5453 : 2000 Standard Test Method for Determination of Total Sulfur in Light Hydrocarbons, Motor Fuels and Oils by Ultraviolet Fluorescence
ASTM D 4809 : 2000 Standard Test Method for Heat of Combustion of Liquid Hydrocarbon Fuels by Bomb Calorimeter (Precision Method)
ASHRAE 79 : 0 Standard Test Method for Determination of Aromatic Hydrocarbon Types in Aviation Fuels and Petroleum Distillates—High Performance Liquid Chromatography Method with Refractive Index Detection
ASTM D 1266 : 1998 Standard Test Method for Sulfur in Petroleum Products (Lamp Method)
ASTM D 4052 : 1996 : R2002 : EDT 1 Standard Test Method for Density and Relative Density of Liquids by Digital Density Meter
ASTM D 1552 : 2008 : R2014 Standard Test Method for Sulfur in Petroleum Products (High-Temperature Method)
ASTM D 1298 : 2012 : REV B Standard Test Method for Density, Relative Density, or API Gravity of Crude Petroleum and Liquid Petroleum Products by Hydrometer Method
ASTM D 5453 : 2004 Standard Test Method for Determination of Total Sulfur in Light Hydrocarbons, Motor Fuels and Oils by Ultraviolet Fluorescence
ASTM D 1319 : 2018 Standard Test Method for Hydrocarbon Types in Liquid Petroleum Products by Fluorescent Indicator Adsorption
ASTM D 5453 : 2012 Standard Test Method for Determination of Total Sulfur in Light Hydrocarbons, Spark Ignition Engine Fuel, Diesel Engine Fuel, and Engine Oil by Ultraviolet Fluorescence
ASTM D 4294 : 2010 Standard Test Method for Sulfur in Petroleum and Petroleum Products by Energy Dispersive X-ray Fluorescence Spectrometry
ASTM D 4052 : 2018 : REV A Standard Test Method for Density, Relative Density, and API Gravity of Liquids by Digital Density Meter
ASTM D 1319 : 2014 Standard Test Method for Hydrocarbon Types in Liquid Petroleum Products by Fluorescent Indicator Adsorption
ASTM D 4809 : 2018 Standard Test Method for Heat of Combustion of Liquid Hydrocarbon Fuels by Bomb Calorimeter (Precision Method)
ASTM D 2887 : 2006 Standard Test Method for Boiling Range Distribution of Petroleum Fractions by Gas Chromatography
ASTM D 1405 : 2001 Standard Test Method for Estimation of Net Heat of Combustion of Aviation Fuels
ASTM D 1552 : 1990 : EDT 1 Standard Test Method for Sulfur in Petroleum Products (High-Temperature Method)
ASTM D 4529 : 2001 : R2006 Standard Test Method for Estimation of Net Heat of Combustion of Aviation Fuels
ASTM D 4809 : 2013 Standard Test Method for Heat of Combustion of Liquid Hydrocarbon Fuels by Bomb Calorimeter (Precision Method)
ASTM D 1405 : 1995 : REV A Standard Test Method for Estimation of Net Heat of Combustion of Aviation Fuels
ASTM D 6379 : 1999 Standard Test Method for Determination of Aromatic Hydrocarbon Types in Aviation Fuels and Petroleum Distillates-High Performance Liquid Chromatography Method with Refractive Index Detection
ASTM D 1266 : 2013 Standard Test Method for Sulfur in Petroleum Products (Lamp Method)
ASTM D 2622 : 1998 Standard Test Method for Sulfur in Petroleum Products by Wavelength Dispersive X-ray Fluorescence Spectrometry
ASTM D 1552 : 2016 Standard Test Method for Sulfur in Petroleum Products by High Temperature Combustion and Infrared (IR) Detection or Thermal Conducitivity Detection (TCD)
ASTM D 1319 : 1999 Standard Test Method for Hydrocarbon Types in Liquid Petroleum Products by Fluorescent Indicator Adsorption
ASTM D 1298 : 1999 : R2005 Standard Test Method for Density, Relative Density (Specific Gravity), or API Gravity of Crude Petroleum and Liquid Petroleum Products by Hydrometer Method
ASTM D 4052 : 2009 Standard Test Method for Density, Relative Density, and API Gravity of Liquids by Digital Density Meter
ASTM D 5453 : 2008 : REV A Standard Test Method for Determination of Total Sulfur in Light Hydrocarbons, Spark Ignition Engine Fuel, Diesel Engine Fuel, and Engine Oil by Ultraviolet Fluorescence
ASTM D 4809 : 2009 : REV A : EDT 1 Standard Test Method for Heat of Combustion of Liquid Hydrocarbon Fuels by Bomb Calorimeter (Precision Method)
ASTM D 1298 : 2012 : REV B : R2017 Standard Test Method for Density, Relative Density, or API Gravity of Crude Petroleum and Liquid Petroleum Products by Hydrometer Method
ASTM D 1298 : 2012 Standard Test Method for Density, Relative Density (Specific Gravity), or API Gravity of Crude Petroleum and Liquid Petroleum Products by Hydrometer Method
ASTM D 1552 : 2000 Standard Test Method for Sulfur in Petroleum Products (High-Temperature Method)
ASTM D 1266 : 1987 Standard Test Method for Sulfur in Petroleum Products (Lamp Method)
ASTM D 2622 : 2008 Standard Test Method for Sulfur in Petroleum Products by Wavelength Dispersive X-ray Fluorescence Spectrometry
ASTM D 4809 : 2000 : R2005 Standard Test Method for Heat of Combustion of Liquid Hydrocarbon Fuels by Bomb Calorimeter (Precision Method)
ASTM D 1298 : 1999 : EDT 2 Standard Test Method for Density, Relative Density (Specific Gravity), or API Gravity of Crude Petroleum and Liquid Petroleum Products by Hydrometer Method
ASTM D 4529 : 2001 : R2011 Standard Test Method for Estimation of Net Heat of Combustion of Aviation Fuels
ASTM D 4529 : 1995 Standard Test Method for Estimation of Net Heat of Combustion of Aviation Fuels
ASTM D 1319 : 2002 : REV A Standard Test Method for Hydrocarbon Types in Liquid Petroleum Products by Fluorescent Indicator Adsorption
ASTM D 5453 : 2005 Standard Test Method for Determination of Total Sulfur in Light Hydrocarbons, Motor Fuels and Oils by Ultraviolet Fluorescence
ASTM D 2887 : 1999 Standard Test Method for Boiling Range Distribution of Petroleum Fractions by Gas Chromatography
ASTM D 2887 : 2006 : REV A Standard Test Method for Boiling Range Distribution of Petroleum Fractions by Gas Chromatography
ASTM D 3120 : 2008 : R2019 Standard Test Method for Trace Quantities of Sulfur in Light Liquid Petroleum Hydrocarbons by Oxidative Microcoulometry
ASTM D 2887 : 2014 Standard Test Method for Boiling Range Distribution of Petroleum Fractions by Gas Chromatography
ASTM D 2887 : 2001 Standard Test Method for Boiling Range Distribution of Petroleum Fractions by Gas Chromatography
ASTM D 1298 : 1999 Standard Test Method for Density, Relative Density (Specific Gravity), or API Gravity of Crude Petroleum and Liquid Petroleum Products by Hydrometer Method
ASTM D 4809 : 1995 Standard Test Method for Heat of Combustion of Liquid Hydrocarbon Fuels by Bomb Calorimeter (Precision Method)
ASTM D 5453 : 2006 Standard Test Method for Determination of Total Sulfur in Light Hydrocarbons, Spark Ignition Engine Fuel, Diesel Engine Fuel, and Engine Oil by Ultraviolet Fluorescence
ASTM D 86 : 2017-06 TEST METHOD FOR DISTILLATION OF PETROLEUM PRODUCTS AND LIQUID FUELS AT ATMOSPHERIC PRESSURE
ASTM D 4294 : 2008 Standard Test Method for Sulfur in Petroleum and Petroleum Products by Energy Dispersive X-ray Fluorescence Spectrometry
ASTM D 3120 : 2003 Standard Test Method for Trace Quantities of Sulfur in Light Liquid Petroleum Hydrocarbons by Oxidative Microcoulometry
ASTM D 4052 : 2011 Standard Test Method for Density, Relative Density, and API Gravity of Liquids by Digital Density Meter
ASTM D 1319 : 2003 Standard Test Method for Hydrocarbon Types in Liquid Petroleum Products by Fluorescent Indicator Adsorption
ASTM D 4052 : 2016 Standard Test Method for Density, Relative Density, and API Gravity of Liquids by Digital Density Meter
ASTM D 4809 : 2009 : REV A Standard Test Method for Heat of Combustion of Liquid Hydrocarbon Fuels by Bomb Calorimeter (Precision Method)
ASTM D 2622 : 2003 Standard Test Method for Sulfur in Petroleum Products by Wavelength Dispersive X-ray Fluorescence Spectrometry
ASTM D 2887 : 2019 : REV A : EDT 1 Standard Test Method for Boiling Range Distribution of Petroleum Fractions by Gas Chromatography
ASTM D 5453 : 2016 Standard Test Method for Determination of Total Sulfur in Light Hydrocarbons, Spark Ignition Engine Fuel, Diesel Engine Fuel, and Engine Oil by Ultraviolet Fluorescence
ASTM D 4294 : 2008 : REV A Standard Test Method for Sulfur in Petroleum and Petroleum Products by Energy Dispersive X-ray Fluorescence Spectrometry
ASTM D 1319 : 2010 Standard Test Method for Hydrocarbon Types in Liquid Petroleum Products by Fluorescent Indicator Adsorption
ASTM D 6379 : 2011 Standard Test Method for Determination of Aromatic Hydrocarbon Types in Aviation Fuels and Petroleum Distillates—High Performance Liquid Chromatography Method with Refractive Index Detection
ASTM D 2887 : 2001 : REV A Standard Test Method for Boiling Range Distribution of Petroleum Fractions by Gas Chromatography
ASTM D 4529 : 2017 : REDLINE Standard Test Method for Estimation of Net Heat of Combustion of Aviation Fuels
ASTM D 4294 : 2002 Standard Test Method for Sulfur in Petroleum Products by Energy-Dispersive X-Ray Fluorescence Spectroscopy
ASTM D 1552 : 2016 : EDT 1 Standard Test Method for Sulfur in Petroleum Products by High Temperature Combustion and Infrared (IR) Detection or Thermal Conductivity Detection (TCD)
ASTM D 3120 : 2003 : REV A Standard Test Method for Trace Quantities of Sulfur in Light Liquid Petroleum Hydrocarbons by Oxidative Microcoulometry
ASTM D 1298 : 1999 : EDT 1 Standard Test Method for Density, Relative Density (Specific Gravity), or API Gravity of Crude Petroleum and Liquid Petroleum Products by Hydrometer Method
ASTM D 1552 : 2007 Standard Test Method for Sulfur in Petroleum Products (High-Temperature Method)
ASTM D 5453 : 2003 Standard Test Method for Determination of Total Sulfur in Light Hydrocarbons, Motor Fuels and Oils by Ultraviolet Fluorescence
ASTM D 1552 : 1995 Standard Test Method for Sulfur in Petroleum Products (High-Temperature Method)
ASTM D 2887 : 2016 Standard Test Method for Boiling Range Distribution of Petroleum Fractions by Gas Chromatography
ASTM D 1552 : 2015 Standard Test Method for Sulfur in Petroleum Products by High Temperature Combustion and IR Detection
ASTM D 1552 : 2001 Standard Test Method for Sulfur in Petroleum Products (High-Temperature Method)
ASTM D 2887 : 2008 Standard Test Method for Boiling Range Distribution of Petroleum Fractions by Gas Chromatography
ASTM D 2887 : 2019 : EDT 1 Standard Test Method for Boiling Range Distribution of Petroleum Fractions by Gas Chromatography
ASTM D 2887 : 2018 Standard Test Method for Boiling Range Distribution of Petroleum Fractions by Gas Chromatography
ASTM D 2887 : 2012 Standard Test Method for Boiling Range Distribution of Petroleum Fractions by Gas Chromatography
ASTM D 4294 : 2016 : EDT 1 Standard Test Method for Sulfur in Petroleum and Petroleum Products by Energy Dispersive X-ray Fluorescence Spectrometry
ASTM D 5453 : 2016 : EDT 1 Standard Test Method for Determination of Total Sulfur in Light Hydrocarbons, Spark Ignition Engine Fuel, Diesel Engine Fuel, and Engine Oil by Ultraviolet Fluorescence
ASTM D 1552 : 2008 : R2014 : EDT 1 Standard Test Method for Sulfur in Petroleum Products (High-Temperature Method)
ASTM D 4294 : 2008 : REV A : EDT 1 Standard Test Method for Sulfur in Petroleum and Petroleum Products by Energy Dispersive X-ray Fluorescence Spectrometry
ASTM D 4052 : 1996 Standard Test Method for Density and Relative Density of Liquids by Digital Density Meter
ASTM D 4294 : 1998 Standard Test Method for Sulfur in Petroleum Products by Energy-Dispersive X-Ray Fluorescence Spectroscopy
ASTM D 4809 : 2009 Standard Test Method for Heat of Combustion of Liquid Hydrocarbon Fuels by Bomb Calorimeter (Precision Method)
ASTM D 2622 : 2010 Standard Test Method for Sulfur in Petroleum Products by Wavelength Dispersive X-ray Fluorescence Spectrometry
ASTM D 4809 : 2006 Standard Test Method for Heat of Combustion of Liquid Hydrocarbon Fuels by Bomb Calorimeter (Precision Method)
ASTM D 2887 : 2019 Standard Test Method for Boiling Range Distribution of Petroleum Fractions by Gas Chromatography
ASTM D 6379 : 2011 : REDLINE TEST METHOD FOR DETERMINATION OF AROMATIC HYDROCARBON TYPES IN AVIATION FUELS AND PETROLEUM DISTILLATES - HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD WITH REFRACTIVE INDEX DETECTION
ASTM D 2887 : 2019 : REV A Standard Test Method for Boiling Range Distribution of Petroleum Fractions by Gas Chromatography
ASTM D 2887 : 2013 Standard Test Method for Boiling Range Distribution of Petroleum Fractions by Gas Chromatography
ASTM D 2887 : 2015 Standard Test Method for Boiling Range Distribution of Petroleum Fractions by Gas Chromatography
ASTM D 5453 : 2019 : REV A Standard Test Method for Determination of Total Sulfur in Light Hydrocarbons, Spark Ignition Engine Fuel, Diesel Engine Fuel, and Engine Oil by Ultraviolet Fluorescence
ASTM D 3120 : 1996 : R2002 : EDT 1 Standard Test Method for Trace Quantities of Sulfur in Light Liquid Petroleum Hydrocarbons by Oxidative Microcoulometry
ASTM D 2887 : 2015 : EDT 1 Standard Test Method for Boiling Range Distribution of Petroleum Fractions by Gas Chromatography
ASTM D 2622 : 2007 Standard Test Method for Sulfur in Petroleum Products by Wavelength Dispersive X-ray Fluorescence Spectrometry
ASTM D 2887 : 2004 Standard Test Method for Boiling Range Distribution of Petroleum Fractions by Gas Chromatography
ASTM D 3120 : 2006 : EDT 1 Standard Test Method for Trace Quantities of Sulfur in Light Liquid Petroleum Hydrocarbons by Oxidative Microcoulometry
ASTM D 2622 : 1994 Standard Test Method for Sulfur in Petroleum Products by X-Ray Spectrometry
ASTM D 3120 : 2006 Standard Test Method for Trace Quantities of Sulfur in Light Liquid Petroleum Hydrocarbons by Oxidative Microcoulometry
ASTM D 6379 : 2004 Standard Test Method for Determination of Aromatic Hydrocarbon Types in Aviation Fuels and Petroleum Distillates—High Performance Liquid Chromatography Method with Refractive Index Detection
ASTM D 1552 : 2008 Standard Test Method for Sulfur in Petroleum Products (High-Temperature Method)
ASTM D 2622 : 2005 Standard Test Method for Sulfur in Petroleum Products by Wavelength Dispersive X-ray Fluorescence Spectrometry
ASTM D 4294 : 2003 Standard Test Method for Sulfur in Petroleum and Petroleum Products by Energy-Dispersive X-Ray Fluorescence Spectrometry
ASTM D 2887 : 2002 Standard Test Method for Boiling Range Distribution of Petroleum Fractions by Gas Chromatography
ASTM D 5453 : 2019 Standard Test Method for Determination of Total Sulfur in Light Hydrocarbons, Spark Ignition Engine Fuel, Diesel Engine Fuel, and Engine Oil by Ultraviolet Fluorescence
ASTM D 1298 : 1985 : R1990 : EDT 1 Standard Practice for Density, Relative Density (Specific Gravity), or API Gravity of Crude Petroleum and Liquid Petroleum Products by Hydrometer Method
ASTM D 1319 : 2015 Standard Test Method for Hydrocarbon Types in Liquid Petroleum Products by Fluorescent Indicator Adsorption
ASTM D 4294 : 2016 Standard Test Method for Sulfur in Petroleum and Petroleum Products by Energy Dispersive X-ray Fluorescence Spectrometry
ASTM D 240 : 2017 : REDLINE Standard Test Method for Heat of Combustion of Liquid Hydrocarbon Fuels by Bomb Calorimeter
ASTM D 2622 : 2016 : REDLINE Standard Test Method for Sulfur in Petroleum Products by Wavelength Dispersive X-ray Fluorescence Spectrometry
ASTM D 1319 : 2013 Standard Test Method for Hydrocarbon Types in Liquid Petroleum Products by Fluorescent Indicator Adsorption
ASTM D 4052 : 2015 Standard Test Method for Density, Relative Density, and API Gravity of Liquids by Digital Density Meter
ASTM D 2887 : 2016 : REV A Standard Test Method for Boiling Range Distribution of Petroleum Fractions by Gas Chromatography
ASTM D 3120 : 2008 : R2014 Standard Test Method for Trace Quantities of Sulfur in Light Liquid Petroleum Hydrocarbons by Oxidative Microcoulometry
ASTM D 2887 : 2004 : REV A Standard Test Method for Boiling Range Distribution of Petroleum Fractions by Gas Chromatography
ASTM D 4529 : 2001 Standard Test Method for Estimation of Net Heat of Combustion of Aviation Fuels
ASTM D 1266 : 2007 Standard Test Method for Sulfur in Petroleum Products (Lamp Method)
ASTM D 1552 : 1983 Standard Test Method for Sulfur in Petroleum Products (High-Temperature Method)
ASTM D 2887 : 2003 Standard Test Method for Boiling Range Distribution of Petroleum Fractions by Gas Chromatography
ASTM D 5453 : 2003 : REV A Standard Test Method for Determination of Total Sulfur in Light Hydrocarbons, Motor Fuels and Oils by Ultraviolet Fluorescence
ASTM D 1319 : 2019 Standard Test Method for Hydrocarbon Types in Liquid Petroleum Products by Fluorescent Indicator Adsorption
ASTM D 4052 : 2018 Standard Test Method for Density, Relative Density, and API Gravity of Liquids by Digital Density Meter
ASTM D 1266 : 2018 Standard Test Method for Sulfur in Petroleum Products (Lamp Method)
ASTM D 4294 : 1990 : R1998 : EDT 1 Standard Test Method for Sulfur in Petroleum Products by Energy-Dispersive X-Ray Fluorescence Spectroscopy
ASTM D 1319 : 2002 Standard Test Method for Hydrocarbon Types in Liquid Petroleum Products by Fluorescent Indicator Adsorption
ASTM D 1405 : 2001 : R2006 Standard Test Method for Estimation of Net Heat of Combustion of Aviation Fuels
ASTM D 2622 : 2016 Standard Test Method for Sulfur in Petroleum Products by Wavelength Dispersive X-ray Fluorescence Spectrometry
ASTM D 5453 : 2008 Standard Test Method for Determination of Total Sulfur in Light Hydrocarbons, Spark Ignition Engine Fuel, Diesel Engine Fuel, and Engine Oil by Ultraviolet Fluorescence
ASTM D 5453 : 2000 : EDT 1 Standard Test Method for Determination of Total Sulfur in Light Hydrocarbons, Motor Fuels and Oils by Ultraviolet Fluorescence
ASTM D 6379 : 2011 : R2019 Standard Test Method for Determination of Aromatic Hydrocarbon Types in Aviation Fuels and Petroleum Distillates—High Performance Liquid Chromatography Method with Refractive Index Detection

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