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ASTM D 4412 : 1984 : R1997

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

View Superseded by

Standard Test Methods for Sulfate-Reducing Bacteria in Water and Water-Formed Deposits

Available format(s)

Hardcopy , PDF

Superseded date

11-11-2014

Language(s)

English

Published date

31-12-2010

€59.22
Excluding VAT

Committee
D 19
DocumentType
Test Method
Pages
3
ProductNote
Reconfirmed 1997
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy

1.1 These test methods cover the procedure for the detection and enumeration by the most probable number (MPN) technique of sulfate-reducing bacteria in water or water-formed deposits.

1.2 Two media preparations are provided. Medium A which is prepared with reagent grade water, and Medium B which is prepared using the water to be sampled as the water source. Medium B is offered for those special conditions where sulfate-reducing bacterial strains have adapted to atypical non-fresh water environment.

1.3 For the isolation and enumeration of thermophilic sulfate-reducing bacteria encountered in waters associated with oil and gas production, all broths, dilution blanks, and incubations must be maintained at tempertures of at least 45°C and preferably within 5° at the sample temperature.

1.4 The sensitivity of these test methods can be increased by purging the dilution blanks and tubes of media with nitrogen immediately prior to use.

1.5 The analyst should be aware that adequate collaborative data for precision and bias statements as required by Practice D2777 are not provided. See Section 11 for details.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

ASTM E 645 : 2018 Standard Practice for Evaluation of Microbicides Used in Cooling Water Systems
ASTM D 6469 : 2020 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
ASTM D 4025 : 2018 Standard Practice for Reporting Results of Examination and Analysis of Deposits Formed from Water for Subsurface Injection
ASTM D 8243 : 2019 Standard Test Method for Determination of APS Reductase to Estimate Sulfate Reducing Bacterial Bioburdens in Water – Enzyme-Linked Immunosorbent Assay Method (Withdrawn 2024)
ASTM D 6469 : 2017 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
ASTM D 887 : 2013 Standard Practices for Sampling Water-Formed Deposits
ASTM D 5978/D5978M : 2016 Standard Guide for Maintenance and Rehabilitation of Groundwater Monitoring Wells

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