BS 6068-4.13(2000) : 2000 AMD 13447
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
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WATER QUALITY - PART 4: MICROBIOLOGICAL METHODS - SECTION 4.13: DETECTION AND ENUMERATION OF BACTERIOPHAGES - ENUMERATION OF SOMATIC COLIPHAGES
19-11-2001
23-11-2012
1 Scope
2 Normative references
3 Terms and definitions
4 Safety precautions
5 Principle
6 Diluent, culture media and reagents
6.1 Basic materials
6.2 Diluent
7 Apparatus and glassware
8 Microbiological reference cultures
9 Sampling
10 Preparation of test material
10.1 Culturing and maintenance of host strains
10.2 Calibration of absorbance measurements for
counts of viable microorganisms
11 Procedure
11.1 Preparation of inoculum cultures
11.2 Standard procedures
11.3 Method for samples with high bacterial
background flora
11.4 Samples with low phage counts
11.5 Presence/absence test
11.6 Quality assurance
12 Expression of results
12.1 Plaque count procedures (11.2 to 11.4)
12.2 Presence/absence test (11.5)
13 Test report
Annex A (normative) Culture media, reagents and diluent
Annex B (informative) General description of somatic
bacteriophages
Annex C (informative) Culturing of bacteriophage phiX174
Bibliography
Defines a procedure for detecting and enumerating somatic coliphages by incubation of the sample with an appropriate host strain. Applies to all types of water, sediments and sludge extracts, following dilution if required. Also applies to shellfish extracts.
| Committee |
EH/3
|
| DevelopmentNote |
Renumbered and superseded by BS EN ISO 10705-2. (12/2001)
|
| DocumentType |
Standard
|
| PublisherName |
British Standards Institution
|
| Status |
Superseded
|
| SupersededBy |
| Standards | Relationship |
| ISO 10705-2:2000 | Identical |
| ISO 5667-2:1991 | Water quality Sampling Part 2: Guidance on sampling techniques |
| ISO 8199:2005 | Water quality General guidance on the enumeration of micro-organisms by culture |
| ISO 5667-3:2012 | Water quality Sampling Part 3: Preservation and handling of water samples |
| ISO 31-0:1992 | Quantities and units Part 0: General principles |
| ISO 5667-1:2006 | Water quality Sampling Part 1: Guidance on the design of sampling programmes and sampling techniques |
| ISO 3696:1987 | Water for analytical laboratory use — Specification and test methods |
| ISO/IEC Guide 2:2004 | Standardization and related activities — General vocabulary |
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