BS EN 12393-2:2013

Foreword
Introduction
1 Scope
2 Normative references
3 Principles
4 General: Summary of procedures
5 Method M: Extraction with acetone and
  liquid-liquid partition with dichloromethane/light
  petroleum, if necessary clean-up on Florisil[R]
6 Method N: Extraction with acetone, liquid-liquid
  partition with dichloromethane or cyclohexane/ethyl
  acetate, clean-up with gel permeation and silica gel
  chromatography
7 Method P: Extraction with ethyl acetate, and if
  necessary, clean-up by gel permeation chromatography
Annex A (informative) - Average water content of
        crops and foods
Bibliography

Describes methods for the extraction and clean-up of food samples of plant origin for quantitative determination of pesticide residues.

This European Standard specifies methods for the extraction and clean-up of food samples of plant origin for quantitative determination of pesticide residues.

Different solvents can be used for this purpose. These pesticide residues are generally associated with other co-extracted compounds which would interfere in the analysis. To purify the crude extracts to be analysed, several techniques can be used.

This European Standard contains the following extraction and clean-up methods that have been subjected to interlaboratory studies and/or are adopted throughout Europe:

• method M: Extraction with acetone and liquid-liquid partition with dichloromethane/light petroleum, if necessary clean-up on Florisil® ¹⁾ [1], [2], [3];

• method N: Extraction with acetone, liquid-liquid partition with dichloromethane or cyclohexane/ethyl acetate and clean-up with gel permeation and silica gel chromatography [4], [5];

• method P: Extraction with ethyl acetate, and if necessary, clean-up by gel permeation chromatography [6].

This European Standard specifies the details of methods M, N and P for the extraction and the clean-up of food samples of plant origin. Several solvents at different volumes are used for extraction. Techniques of clean-up are listed such as liquid-liquid partition, liquid chromatography on various adsorbents and gel permeation chromatography.

A table providing the couples (matrix/pesticide) which have been submitted to collaborative studies and a list of indicative applicability of the method to different pesticides are given for each method, wherever possible.