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BS EN ISO 12966-2:2017

Current

Current

The latest, up-to-date edition.

Animal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters Preparation of methyl esters of fatty acids

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

11-08-2017

€217.36
Excluding VAT

Committee
AW/307
DevelopmentNote
Supersedes BS EN ISO 5509, BS 684-2.34(2001) and 09/30190499 DC. (03/2011)
DocumentType
Standard
Pages
28
PublisherName
British Standards Institution
Status
Current
Supersedes

This document specifies methods of preparing the methyl esters of fatty acids. It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements, four methylation methods are specified, namely: a “rapid” transmethylation procedure under alkaline conditions; a “general” transmethylation/methylation procedure under sequential alkaline and acid conditions; a BF3 transmethylation procedure; an alternative procedure using acid-catalysed transmethylation of glycerides. Methyl esters so produced are used in various analytical procedures requiring such derivatives, e.g. gas-liquid chromatography (GLC), thin-layer chromatography (TLC) and infrared spectrometry (IR). Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.

Standards Relationship
EN ISO 12966-2:2017 Identical
ISO 12966-2:2017 Identical

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ISO 4799:1978 Laboratory glassware — Condensers
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ISO 3696:1987 Water for analytical laboratory use — Specification and test methods
ISO 661:2003 Animal and vegetable fats and oils — Preparation of test sample
ISO 78-2:1999 Chemistry Layouts for standards Part 2: Methods of chemical analysis
ISO 4800:1998 Laboratory glassware — Separating funnels and dropping funnels

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