BS EN ISO 14240-2:2011
Current
The latest, up-to-date edition.
Soil quality. Determination of soil microbial biomass Fumigation-extraction method
30-09-2011
Introduction
1 Scope
2 Normative references
3 Definition
4 Principle
5 Materials and reagents
6 Apparatus
7 Fumigation and extraction
8 Determination of carbon in the extracts
9 Precision
Annex A (normative) - Measurement of water-holding
capacity of soil
Annex B (informative) - Estimation of soil microbial
biomass in soils containing large amounts of
living roots - Pre-extraction procedure
Annex C (informative) - Results of an interlaboratory
trial
Annex D (informative) - Bibliography
Describes a method for the estimation of microbial biomass of soils by measurement of total extractable organic biomass material mainly from freshly killed microorganisms.
| Committee |
EH/4
|
| DevelopmentNote |
Renumbers & supersedes BS 7755-4.4.2(1997). 2011 version incorporates corrigendum to BS 7755-4.4.2(1997). (09/2011)
|
| DocumentType |
Standard
|
| PublisherName |
British Standards Institution
|
| Status |
Current
|
| Supersedes |
This part of ISO 14240 specifies a method for the estimation of microbial biomass of soils by measurement of total extractable organic biomass material mainly from freshly killed microorganisms. The method is also applicable to the estimation of microbial nitrogen and microbial ninhydrin-reactive nitrogen in soil, but this part of ISO 14240 describes only the measurement of extractable organic carbon. The fumigation-extraction (FE) method is applicable to aerobic and anaerobic (water-logged, paddy) soils overthe whole range of soil pH. Biomass can be also measured in soils containing actively decomposing substrates and soils supersaturated with potassium sulfate solution. NOTE Chloroform fumigation also affects soil fauna. The contribution of carbon from these organisms is generally small (< 5 %) and can usually be neglected.
| Standards | Relationship |
| EN ISO 14240-2:2011 | Identical |
| ISO 14240-2:1997 | Identical |
| ISO 10381-6:2009 | Soil quality Sampling Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory |
| ISO 11274:1998 | Soil quality Determination of the water-retention characteristic Laboratory methods |
| ISO 10390:2005 | Soil quality Determination of pH |
| ISO 10694:1995 | Soil quality — Determination of organic and total carbon after dry combustion (elementary analysis) |
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