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BS EN ISO 17601:2018

Current

Current

The latest, up-to-date edition.

Soil quality. Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil

Published date

22-03-2018

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Committee
EH/4
DevelopmentNote
2018 Edition incorporates corrigendum to BS ISO 17601. Renumbers and supersedes BS ISO 17601. Supersedes 13/30253328 DC. (03/2018)
DocumentType
Standard
PublisherName
British Standards Institution
Status
Current

This International Standard specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups.

It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies in different bacterial phyla. Therefore, the number of 16S rRNA sequences quantified from soil DNA extracts does not give an exact estimate of the number of soil bacteria. Furthermore, the number of sequences is not necessarily linked to living microorganisms and can comprise sequences amplified from dead microorganisms.

Standards Relationship
ISO 17601:2016 Identical
EN ISO 17601:2018 Identical

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