BS EN ISO 21871:2006
Current
The latest, up-to-date edition.
Microbiology of food and animal feeding stuffs. Horizontal method for the determination of low numbers of presumptive Bacillus cereus. Most probable number technique and detection method
Hardcopy , PDF
English
01-02-2006
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 Enumeration method
4.2 Detection method
5 Diluent, culture media and reagents
5.1 General
5.2 Diluent
5.3 Liquid selective enrichment medium:
Tryptone soya polymyxin broth (TSPB)
5.4 Solid selective medium: Polymyxin
pyruvate egg yolk mannitol bromothymol
blue agar (PEMBA)
5.5 Solid selective medium: Mannitol egg
yolk polymyxin agar (MYP)
5.6 Staining solutions for microscopic
identification
5.7 Sheep blood agar
6 Apparatus and glassware
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Enumeration method
9.2 Detection method
10 Calculation and expression of results
10.1 Enumeration method for the determination
of the most probable number (MPN)
10.2 Detection method
11 Test report
Annex A (normative) Diagram of enumeration procedure
Bibliography
Describes a horizontal method for the detection or the enumeration of low numbers of viable presumptive Bacillus cereus by means of the most probable number technique.
| Committee |
AW/9
|
| DevelopmentNote |
Supersedes 04/30110927 DC. (02/2006)
|
| DocumentType |
Standard
|
| Pages |
26
|
| PublisherName |
British Standards Institution
|
| Status |
Current
|
| Supersedes |
This International Standard specifies a horizontal method for the detection or the enumeration of low numbers of viable presumptive Bacillus cereus by means of the most probable number technique. This International Standard is applicable to
-
products intended for human consumption and the feeding of animals, and
-
environmental samples in the area of food production and food handling.
| Standards | Relationship |
| EN ISO 21871:2006 | Identical |
| UNE-EN ISO 21871:2007 | Identical |
| NEN EN ISO 21871 : 2006 | Identical |
| NBN EN ISO 21871 : 2006 | Identical |
| UNI EN ISO 21871 : 2006 | Identical |
| I.S. EN ISO 21871:2006 | Identical |
| ISO 21871:2006 | Identical |
| DIN EN ISO 21871:2006-04 | Identical |
| SN EN ISO 21871 : 2006 | Identical |
| NF EN ISO 21871 : 2006 | Identical |
| NS EN ISO 21871 : 1ED 2006 | Identical |
| ISO/TS 11133-1:2009 | Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 1: General guidelines on quality assurance for the preparation of culture media in the laboratory |
| ISO 7218:2007 | Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations |
| ISO/TS 11133-2:2003 | Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 2: Practical guidelines on performance testing of culture media |
| ISO 8261:2001 | Milk and milk products General guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination |
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