BS ISO 4831:2006

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Culture media and diluents
6 Apparatus and glassware
7 Sampling
8 Preparation of the test sample
9 Procedure
10 Calculation and expression of results
11 Precision
12 Test report
Annex A (normative) Flowcharts for procedure
Bibliography

Provides general guidelines for the detection and the enumeration of coliforms.

This International Standard gives general guidelines for the detection and the enumeration of coliforms. It is applicable to

• products intended for human consumption and for the feeding of animals, and

• environmental samples in the area of food production and food handling.

Enumeration is carried out by calculation of the most probable number (MPN) after incubation in a liquid medium at 30 °C or 37 °C.

NOTE The temperature is subject to agreement between the parties concerned. In the case of milk and milk products, the temperature of incubation is 30 °C.

This enumeration method is applicable when the number sought is expected to be in the range 1 to 100 per millilitre or per gram of test sample.

A limitation on the applicability of this International Standard is imposed by the susceptibility of the method to a large degree of variability. The information given in Clause 11 provides guidance on the applicability of the method and on the interpretation of the results.