CEN ISO/TS 18867:2015

European foreword
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principles
5 Reagents
6 Apparatus and equipment
7 Sampling
8 Procedure
9 Test report
Annex A (normative) - PCR detection and isolation of
        pathogenic Y. enterocolitica
Annex B (informative) - Real-time PCR for detection
        of Y. enterocolitica
Annex C (informative) - Detection and isolation of
        Y. pseudotuberculosis
Annex D (informative) - Simultaneous detection of
        pathogenic Y. enterocolitica and Y. pseudotuberculosis
        using multiplex real-time PCR
Bibliography

ISO/TS 18867:2015 specifies two horizontal methods for detection of the pathogenic bioserotypes of Y. enterocolitica and one for detection of Y. pseudotuberculosis by using real-time PCR-based methods. The described methods allow for the detection of the two pathogens in enrichments and allow the isolation of colonies. Y. pestis, the causative agent of bubonic and pneumonic plague harbours a variant of the ail gene as well and will be detected by the same primer/probe set as Y. pseudotuberculosis. However, Y. pestis is normally not associated with food. This Technical Specification is applicable to products for human consumption, animal feeding stuffs, and environmental samples.