CLSI I/LA26 A2 : 2ED 2013
Current
The latest, up-to-date edition.
PERFORMANCE OF SINGLE CELL IMMUNE RESPONSE ASSAYS
Hardcopy , PDF
English
27-11-2013
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Abstract
Committee Membership
Foreword
1 Scope
2 Standard Precautions
3 Terminology
4 Specimen Collection
5 Specimen Transport and Handling
6 Sample Preparation
7 Laboratory Procedure for the Assessment of
Antigen-Specific Cellular Immune Responses Using
Intracellular Cytokine Staining
8 Laboratory Procedure for the Assessment of
Antigen-Specific Cellular Immune Responses Using
Major Histocompatibility Complex Multimers
9 Laboratory Procedure for the Assessment of
Antigen-Specific Cellular Immune Responses Using
Enzyme-Linked Immunospot Technology
10 Laboratory Procedure for the Assessment of
Antigen-Specific Cellular Immune Responses Using
the Tracking Dye Carboxyfluorescein Succinimidyl Ester
11 Quality Assurance and Test Validation
12 Data Reporting
References
Appendix A - Cryopreservation of Viable Cells
Appendix B - Statistics of Rare-Event Analyses
Appendix C - Troubleshooting Table for Antigen-Specific
Intracellular Cytokine Staining Assay
Appendix D - Troubleshooting Table for Major Histocompatibility
Complex Multimer Assay
Appendix E - Troubleshooting Table for Enzyme-Linked
Immunospot Assays
Appendix F - Troubleshooting Table for Carboxyfluorescein
Succinimidyl Ester
The Quality Management System Approach
Related CLSI Reference Materials
This document provides guidance for the performance of single cell immune response assays within the clinical context of infectious diseases (especially HIV), cancer, transplantation, autoimmune disease, and allergies.
| DevelopmentNote |
Supersedes CLSI I/LA26 A. (12/2013)
|
| DocumentType |
Miscellaneous Product
|
| ISBN |
1-56238-893-2
|
| Pages |
106
|
| PublisherName |
Clinical Laboratory Standards Institute
|
| Status |
Current
|
| Supersedes |
| CLSI EP14 A2 : 2ED 2005 | EVALUATION OF MATRIX EFFECTS |
| CLSI GP29 A2 : 2ED 2008 | ASSESSMENT OF LABORATORY TESTS WHEN PROFICIENCY TESTING IS NOT AVAILABLE |
| CLSI M29 A3 : 3ED 2005 | ACQUIRED INFECTIONS; APPROVED GUIDELINE |
| CLSI H42 A2 : 2ED 2007 | ENUMERATION OF IMMUNOLOGICALLY DEFINED CELL POPULATIONS BY FLOW CYTOMETRY |
| CLSI GP41 A6 : 6ED 2007 | PROCEDURES FOR THE COLLECTION OF DIAGNOSTIC BLOOD SPECIMENS BY VENIPUNCTURE |
| CLSI QMS02 A6 : 6ED 2013 | QUALITY MANAGEMENT SYSTEM: DEVELOPMENT AND MANAGEMENT OF LABORATORY DOCUMENTS |
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