CLSI I/LA26 A2 : 2ED 2013
Current
The latest, up-to-date edition.
PERFORMANCE OF SINGLE CELL IMMUNE RESPONSE ASSAYS
Hardcopy , PDF
English
27-11-2013
Abstract
Committee Membership
Foreword
1 Scope
2 Standard Precautions
3 Terminology
4 Specimen Collection
5 Specimen Transport and Handling
6 Sample Preparation
7 Laboratory Procedure for the Assessment of
Antigen-Specific Cellular Immune Responses Using
Intracellular Cytokine Staining
8 Laboratory Procedure for the Assessment of
Antigen-Specific Cellular Immune Responses Using
Major Histocompatibility Complex Multimers
9 Laboratory Procedure for the Assessment of
Antigen-Specific Cellular Immune Responses Using
Enzyme-Linked Immunospot Technology
10 Laboratory Procedure for the Assessment of
Antigen-Specific Cellular Immune Responses Using
the Tracking Dye Carboxyfluorescein Succinimidyl Ester
11 Quality Assurance and Test Validation
12 Data Reporting
References
Appendix A - Cryopreservation of Viable Cells
Appendix B - Statistics of Rare-Event Analyses
Appendix C - Troubleshooting Table for Antigen-Specific
Intracellular Cytokine Staining Assay
Appendix D - Troubleshooting Table for Major Histocompatibility
Complex Multimer Assay
Appendix E - Troubleshooting Table for Enzyme-Linked
Immunospot Assays
Appendix F - Troubleshooting Table for Carboxyfluorescein
Succinimidyl Ester
The Quality Management System Approach
Related CLSI Reference Materials
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