CLSI M31 A3 : 3ED 2008

Abstract
Committee Membership
Foreword
1 Scope
2 Introduction
3 Definitions
4 Indications for Performing Susceptibility Testing
   4.1 Limitations of Disk Diffusion and Dilution Methods
5 Selection of Antimicrobial Agents
   5.1 Nonproprietary Names
   5.2 Selection Guidelines
   5.3 Antimicrobial Classes
   5.4 Guidelines for Routine Reporting
   5.5 Guidelines for Selective Reporting
   5.6 Interpretive Categories
6 Disk Diffusion Susceptibility Tests
   6.1 Equivalent MIC Breakpoints
   6.2 Methodologies
   6.3 Streptococcus spp.
   6.4 Pasteurella multocida and Mannheimia haemolytica
   6.5 Other Organisms
   6.6 Campylobacter jejuni and Related Species
   6.7 Anaerobic Organisms
   6.8 Detection of Resistant Staphylococci
   6.9 Detection of Resistant Enterococci
7 Broth and Agar Dilution Susceptibility Testing
   7.1 Indications for Performing Broth and Agar Dilution
        Susceptibility Tests
8 Antimicrobial Agents
   8.1 Source
   8.2 Weighing Antimicrobial Agent Powders
   8.3 Preparing Stock Solutions
   8.4 Number of Concentrations Tested
9 Agar Dilution Procedure
   9.1 Reagents and Materials
   9.2 Agar Dilution Testing of C. jejuni
10 Broth Dilution Procedure
   10.1 Broth Medium
   10.2 Cation Adjustments
   10.3 Preparing and Storing Diluted Antimicrobial Agents
   10.4 Standardizing Inoculum for Broth Dilution Testing
   10.5 Inoculating Broth
   10.6 Incubation
   10.7 Interpreting Results
11 Broth Dilution Susceptibility Testing of Fastidious
   Organisms
   11.1 P. multocida and M. haemolytica
   11.2 Broth Microdilution Testing of H. somni and
        A. pleuropneumoniae
   11.3 Broth Microdilution Testing of Campylobacter spp.
   11.4 Streptococci and Listeria spp.
   11.5 Staphylococcus hyicus
12 Detection of Resistant Staphylococci
   12.1 Methicillin/Oxacillin Resistance
   12.2 Oxacillin Screening Plates
   12.3 Reduced Susceptibility to Vancomycin
13 Detection of Resistant Enterococci
   13.1 Penicillin-Ampicillin Resistance
   13.2 Vancomycin Resistance
   13.3 High-Level Aminoglycoside Resistance
14 QC Guidelines
   14.1 Purpose
   14.2 QC Responsibilities
   14.3 Suggested Reference Strains
   14.4 Disk Diffusion Tests
   14.5 Frequency of QC Testing (also refer to Appendix B)
   14.6 Dilution Susceptibility Tests
   14.7 Daily Testing
   14.8 Weekly Testing
   14.9 Corrective Action
15 Specific Antimicrobial Resistance Tests
   15.1 Purpose
   15.2 beta-Lactamase Tests
   15.3 Selecting a beta-Lactamase Test
   15.4 Detection of Extended-Spectrum,
        beta-Lactamase-Producing Enterobacteriaceae
16 Cumulative Antimicrobial Susceptibility Profile
References
Appendix A. Antimicrobial Susceptibility QC Record
Appendix B1. Disk Diffusion Daily QC Testing Protocol
Appendix B2. Disk Diffusion Weekly QC Testing Protocol
Appendix C1. Aerobic Dilution Daily QC Testing Protocol
Appendix C2. Aerobic Dilution Weekly QC Testing Protocol
Appendix D1. Disk Diffusion QC Troubleshooting Guide
Appendix D2. MIC QC Troubleshooting Guide

Provides the currently recommended techniques for antimicrobial agent disk and dilution susceptibility testing, criteria for quality control testing, and interpretive criteria for veterinary use.