CLSI M7 A7 : 7ED 2006

Abstract
Committee Membership
Foreword
Summary of Major Changes in This Document
Subcommittee on Antimicrobial Susceptibility Testing
Mission Statement
1 Scope
2 Introduction
3 Standard Precautions
4 Definitions
5 Indications for Performing Susceptibility Tests
6 Antimicrobial Agents
   6.1 Source
   6.2 Weighing Antimicrobial Powders
   6.3 Preparing Stock Solutions
   6.4 Number of Concentrations Tested
7 Selection of Antimicrobial Agents for Routine
   Testing and Reporting
   7.1 Routine Reports
   7.2 Nonproprietary Names
   7.3 Selection Guidelines
   7.4 Suggested Guidelines for Routine and Selective
         Testing and Reporting
8 Inoculum Preparation for Dilution Tests
   8.1 Turbidity Standard for Inoculum Preparation
   8.2 Direct Colony Suspension Method
   8.3 Growth Method
9 Agar Dilution Procedure
   9.1 Reagents and Materials
   9.2 Preparing Agar Dilution Plates
   9.3 Preparing the Inoculum
   9.4 Inoculating Agar Dilution Plates
   9.5 Incubating Agar Dilution Plates
   9.6 Determining Agar Dilution End Points
10 Broth Dilution Procedures (Macrodilution and
   Microdilution)
   10.1 Mueller-Hinton Broth
   10.2 Preparing and Storing Diluted Antimicrobial
         Agents
   10.3 Broth Dilution Testing
11 Fastidious Organisms
   11.1 Haemophilus influenzae and H. parainfluenzae
   11.2 Neisseria gonorrhoeae
   11.3 Neisseria meningitidis
   11.4 Streptococcus pneumoniae and Other
         Streptococcus spp
12 Problem Organisms
   12.1 Staphylococci
   12.2 Enterococci
   12.3 Extended-Spectrum, a-Lactamase-Producing,
         Gram-Negative Bacilli
13 Inducible Clindamycin Resistance
14 a-Lactamase Tests
   14.1 Purpose
   14.2 Selecting a a-Lactamase Test
15 Reporting of MIC Results
   15.1 Susceptible
   15.2 Intermediate
   15.3 Resistant
16 Quality Control Procedures
   16.1 Purpose
   16.2 Quality Control Responsibilities
   16.3 Reference Strains for Quality Control
   16.4 Storing and Testing Quality Control Strains
   16.5 Batch or Lot Quality Control
   16.6 MIC Quality Control Limits
   16.7 Frequency of Quality Control Testing
   16.8 Frequency of Quality Control Testing for
         Screening Tests
   16.9 Corrective Action
   16.10 Reporting Patient Results When Out-of-Control
         Tests Occur
   16.11 Verification of Patient Test Results
   16.12 Other Control Procedures
17 Limitations of Dilution Test Methods
   17.1 Application to Various Organism Groups
   17.2 Misleading Results
   17.3 Emergence of Resistance
18 Screening Tests
References
Appendix A - Quality Control Protocol Flow Charts
Summary of Comments and Subcommittee Responses
Summary of Delegate Comments and Subcommittee Responses
The Quality System Approach
Related CLSI/NCCLS Publications

Describes the standard broth (macrodilution and microdilution) and agar dilution methods used to determine the in vitro susceptibility of bacteria that grow aerobically.