CLSI VET03 A : 1ED 2006

Abstract
Committee Membership
Foreword
1 Scope
2 Introduction
3 Definitions
4 Indications for Performing Susceptibility Testing
5 Selection of Antimicrobial Agents for Routine Testing
  and Reporting
  5.1 Routine Reports
  5.2 Antimicrobial Classes
  5.3 Suggested Guidelines for Use and Selective Testing
      and Reporting
6 Disk Diffusion Assay Protocol
  6.1 Media Preparation
  6.2 Inoculum
  6.3 Diffusion Disks
  6.4 Incubation
  6.5 Reading Plates
  6.6 Rejection Criteria
7 Fastidious and Problem Organisms
  7.1 Vibrionaceae and Photobacteriaceae (Obligate Halophilic
      Strains) (Group 2)
  7.2 Gliding Bacteria (Group 3)
  7.3 Streptococci (Group 4)
  7.4 Other Fastidious Organisms (Group 5)
8 Quality Control Procedures
  8.1 Purpose
  8.2 Reference Strains for Quality Control
  8.3 Accepted Quality Control Ranges
  8.4 Expansion of Control Strain Set
  8.5 Storing and Testing Quality Control Strains
  8.6 Control of Media and
  8.7 Frequency of Quality Control Testing
  8.8 Corrective Action
9 Quality Assurance Measures
References
Appendix A - Antimicrobial Agents Used in Global Aquaculture
             and Status of Quality Control for Disk Diffusion
             Susceptibility Testing
Appendix B - Fuzzy Zones and Halo Formation
Appendix C - Disk Diffusion Daily Quality Control Testing
             Protocol
Appendix D - Disk Diffusion Weekly Quality Control Testing
             Protocol
Summary of Delegate/Consensus Comments and Working Group
Responses
The Quality System Approach
Related CLSI/NCCLS Publications

Provides the most up-to-date techniques for disk diffusion susceptibility testing of aquatic species isolates, and criteria for quality control testing.

This CLSI guideline provides veterinary and aquatic animal disease diagnostic laboratories with currently recommended antimicrobial disk diffusion susceptibility testing methods for bacteria isolated from aquatic animals–primarily Group 1 organisms–including criteria for quality control testing with two quality control strains. The document also provides appendixes and tables outlining recommended disk concentrations, antimicrobial agents used in global aquaculture, methods for preparing stock solutions and dilutions of antimicrobial agents, and a list of bacteria pathogenic to fish.Interpretive criteria are not addressed in this guideline. Such criteria must be established using pharmacokinetic and pharmacodynamic data, in vitro susceptibility testing data, and clinical efficacy data. Developing interpretive criteria was beyond the scope of this document. As more aquatic animal-specific information becomes available, this document, and CLSI document M49—Methods for Broth Dilution Susceptibility Testing of Bacteria Isolated From Aquatic Animals, will be revised to incorporate those data.