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CLSI VET04 A : 1ED 2006

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

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METHODS FOR BROTH DILUTION SUSCEPTIBILITY TESTING OF BACTERIA ISOLATED FROM AQUATIC ANIMALS

Superseded date

25-09-2014

Superseded by

CLSI VET04 A2 : 2ED 2014

Published date

12-01-2013

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Abstract
Committee Membership
Foreword
1 Scope
2 Introduction
3 Definitions
4 Antimicrobial Agents
   4.1 Source
   4.2 Weighing Antimicrobial Powders
   4.3 Preparing Stock Solutions
   4.4 Number of Concentrations Tested
5 Selection of Antimicrobial Agents for Routine Testing
   and Reporting
   5.1 Routine Reports
   5.2 Antimicrobial Classes
   5.3 Suggested Guidelines for Use and Selective Testing
        and Reporting
6 Indications for Performing Susceptibility Testing
7 Inoculum Preparation for Dilution Tests
   7.1 Turbidity Standard for Inoculum Preparation
   7.2 Direct Colony Suspension Method
8 Broth Dilution Procedures (Macrodilution and Microdilution)
   8.1 Cation-Adjusted Mueller-Hinton Broth (CAMHB) Medium
   8.2 Preparing and Storing Diluted Antimicrobial Agents
   8.3 Broth Dilution Testing
9 Fastidious and Problem Organisms
   9.1 Vibrionaceae and Photobacteriaceae (Obligate Halophilic
        Strains) (Group 2)
   9.2 Gliding Bacteria (Group 3)
   9.3 Streptococci (Group 4)
   9.4 Other Fastidious Organisms (Group 5)
10 Reporting of MIC Results
11 Quality Control Procedures
   11.1 Purpose
   11.2 Quality Control Responsibilities
   11.3 Reference Strains for Quality Control
   11.4 Storing and Testing Quality Control Strains
   11.5 Control of Media and Materials
   11.6 Broth Dilution Quality Control Ranges
   11.7 Frequency of Quality Control Testing
   11.8 Corrective Action
   11.9 Other Control Procedures
12 Limitations of Broth Dilution Test Methods
   12.1 Application to Various Organism Groups
References
Appendix A - Antimicrobial Agents Used in Global Aquaculture
             and Status of Quality Control for Broth Dilution
             Susceptibility Testing
Appendix B - Aerobic Dilution Daily Quality Control Testing
             Protocol
Appendix C - Aerobic Dilution Weekly Quality Control Testing
             Protocol
Summary of Comments and Subcommittee Responses
The Quality System Approach
Related CLSI/NCCLS Publications

Provides the most up-to-date techniques for the determination of minimal inhibitory concentrations (MICs) of aquatic bacteria by broth micro- and macrodilution, and criteria for quality control testing.

DevelopmentNote
Supersedes NCCLS M49 P (07/2006) Formerly CLSI M49 A. (07/2013)
DocumentType
Miscellaneous Product
PublisherName
Clinical Laboratory Standards Institute
Status
Superseded
SupersededBy
Supersedes

CLSI VET05 R : 1ED 2011 GENERATION, PRESENTATION, AND APPLICATION OF ANTIMICROBIAL SUSCEPTIBILITY TEST DATA FOR BACTERIA OF ANIMAL ORIGIN
CLSI VET01 A4 : 4ED 2013 PERFORMANCE STANDARDS FOR ANTIMICROBIAL DISK AND DILUTION SUSCEPTIBILITY TESTS FOR BACTERIA ISOLATED FROM ANIMALS
CLSI VET03/VET04 S2 : 2ED 2014 PERFORMANCE STANDARDS FOR ANTIMICROBIAL SUSCEPTIBILITY TESTING OF BACTERIA ISOLATED FROM AQUATIC ANIMALS
CLSI VET02 A3 : 3ED 2008 DEVELOPMENT OF IN VITRO SUSCEPTIBILITY TESTING CRITERIA AND QUALITY CONTROL PARAMETERS FOR VETERINARY ANTIMICROBIAL AGENTS

CLSI M23 A2 : 2ED 2001 DEVELOPMENT OF IN VITRO SUSPECTIBILITY TESTING CRITERIA AND QUALITY CONTROL PARAMETERS
CLSI M6 A2 : 2ED 2006 PROTOCOLS FOR EVALUATING DEHYDRATED MUELLER-HINTON AGAR
CLSI M7 A7 : 7ED 2006 METHODS FOR DILUTION ANTIMICROBIAL SUSCEPTIBILITY TESTS FOR BACTERIA THAT GROW AEROBICALLY
CLSI VET03 A : 1ED 2006 METHODS FOR ANTIMICROBIAL DISK SUSCEPTIBILITY TESTING OF BACTERIA ISOLATED FROM AQUATIC ANIMALS
CLSI M11 A6 : 6ED 2004 METHODS FOR ANTIMICROBIAL SUSCEPTIBILITY TESTING OF ANAEROBIC BACTERIA
CLSI M2 A9 : 9ED 2006 PERFORMANCE STANDARDS FOR ANTIMICROBIAL DISK SUSCEPTIBILITY TESTS

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