DD ISO/TS 21872-1:2007
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
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Microbiology of food and animal feeding stuffs. Horizontal method for the detection of potentially enteropathogenic Vibrio spp. Detection of Vibrio parahaemolyticus and Vibrio cholerae
Hardcopy , PDF
30-09-2017
English
31-12-2008
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 General
4.2 First enrichment in a liquid selective
medium
4.3 Second enrichment in a liquid selective
medium
4.4 Isolation and identification
4.5 Confirmation
5 Culture media and reagents
5.1 Enrichment medium: Alkaline saline
peptone water (ASPW)
5.2 Solid selective isolation media
5.3 Saline nutrient agar (SNA)
5.4 Reagent for detection of oxidase
5.5 Saline triple sugar iron (TSI) agar
5.6 Saline medium for detection of ornithine
decarboxylase (ODC)
5.7 Saline medium for detection of lysine
decarboxylase (LDC)
5.8 Saline medium for detection of arginine
dihydrolase (ADH)
5.9 Reagent for detection of beta-galactosidase
5.10 Saline medium for detection of indole
5.11 Saline peptone waters
5.12 Sodium chloride solution
6 Apparatus and glassware
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Test portion and initial suspension
9.2 First selective enrichment
9.3 Second selective enrichment
9.4 Isolation and identification
9.5 Confirmation
10 Expression of results
11 Test report
Annex A (normative) Diagram of procedure
Annex B (normative) Composition and preparation of
the culture media and reagents
Bibliography
Defines a horizontal method for the detection of the two main pathogenic Vibrio species causing intestinal illness in humans: V. parahaemolyticus and V. cholerae.
| Committee |
AW/9
|
| DevelopmentNote |
Supersedes BS 5763-14(1991). (03/2010)
|
| DocumentType |
Standard
|
| Pages |
28
|
| PublisherName |
British Standards Institution
|
| RevisionOf | |
| Status |
Superseded
|
| SupersededBy | |
| Supersedes |
| Standards | Relationship |
| ISO/TS 21872-1:2007 | Identical |
| ISO/TS 11133-1:2009 | Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 1: General guidelines on quality assurance for the preparation of culture media in the laboratory |
| ISO 7218:2007 | Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations |
| ISO/TS 11133-2:2003 | Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 2: Practical guidelines on performance testing of culture media |
| ISO 8261:2001 | Milk and milk products General guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination |
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