• EN 13098:2000

    Superseded A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

    Workplace atmosphere - Guidelines for measurement of airborne micro-organisms and endotoxin

    Available format(s): 

    Superseded date:  12-09-2019

    Language(s): 

    Published date:  20-09-2000

    Publisher:  Comite Europeen de Normalisation

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    Table of Contents - (Show below) - (Hide below)

    Foreword
    Introduction
    1 Scope
    2 Normative references
    3 Terms and definitions
    4 Symbols and abbreviations
    5 Measurement of micro-organisms and endotoxin
    6 Sampling
    7 Analytical method
    8 Expression of results
    9 Report
    Annex A (informative) Recommendations for selection of
            measurement methods
    Annex B (informative) Example of sampling form
    Annex C (informative) List of general media
    Annex D (informative) Colony counting - Formula and
            examples
    Bibliography

    Abstract - (Show below) - (Hide below)

    The European Standard provides guidelines for the assessment of workplace exposure to airborne micro-organisms including the determination of total number and culturable number of micro-organisms in the workplace atmosphere. The Standard also provides methods for measurement of airborne endotoxin in the work environment. The European Standard does not apply to viruses, specific pathogenic micro-organisms and toxins other than endotoxin, although some of themeasurement principles may be the same.

    General Product Information - (Show below) - (Hide below)

    Committee CEN/TC 137
    Development Note Supersedes PREN 13098 (10/2005)
    Document Type Standard
    Publisher Comite Europeen de Normalisation
    Status Superseded
    Superseded By

    Standards Referenced By This Book - (Show below) - (Hide below)

    10/30196970 DC : 0 BS ISO 16000-19 - INDOOR AIR - PART 19: SAMPLING STRATEGY FOR MOULDS
    ISO 16000-21:2013 Indoor air — Part 21: Detection and enumeration of moulds — Sampling from materials
    BS ISO 16000-20:2014 Indoor air Detection and enumeration of moulds. Determination of total spore count
    06/30146347 DC : 0 ISO 16000-17 - INDOOR AIR - PART 17: DETECTION AND ENUMERATION OF MOULDS - CULTURE-BASED METHOD
    BS ISO 16000-16:2008 Indoor air Detection and enumeration of moulds. Sampling by filtration
    BS ISO 16000-21:2013 Indoor air Detection and enumeration of moulds. Sampling from materials
    BS ISO 16000-19 : 2012 INDOOR AIR - PART 19: SAMPLING STRATEGY FOR MOULDS
    EN 14031:2003 Workplace atmospheres - Determination of airborne endotoxins
    BS EN 14031:2003 Workplace atmospheres. Determination of airborne endotoxins
    ISO 16000-20:2014 Indoor air Part 20: Detection and enumeration of moulds Determination of total spore count
    UNE-EN ISO 16000-19:2015 Indoor air - Part 19: Sampling strategy for moulds (ISO 16000-19:2012)
    CEN/TS 16115-1:2011 Ambient air quality - Measurement of bioaerosols - Part 1: Determination of moulds using filter sampling systems and culture-based analyses
    CEN/TS 16115-2:2016 Ambient air - Measurement of bioaerosols - Part 2: Planning and evaluation of plant-related plume measurements
    14/30273490 DC : 0 BS ISO 16000-20 - INDOOR AIR - PART 20: DETECTION AND ENUMERATION OF MOULDS - DETERMINATION OF TOTAL SPORE COUNT
    02/564413 DC : DRAFT OCT 2002 PREN 14583 - WORKPLACE ATMOSPHERES - VOLUMETRIC BIOAEROSOL SAMPLING DEVICES - REQUIREMENTS AND TEST METHODS
    UNI CEN/TS 16115-1 : 2011 AMBIENT AIR QUALITY - MEASUREMENT OF BIOAEROSOLS - PART 1: DETERMINATION OF MOULDS USING FILTER SAMPLING SYSTEMS AND CULTURE-BASED ANALYSES
    DIN ISO 16000-20 E : 2015 INDOOR AIR - PART 20: DETECTION AND ENUMERATION OF MOULDS - DETERMINATION OF TOTAL SPORE COUNT (ISO 16000-20:2014)
    DD CEN/TS 16115-1:2011 Ambient air quality. Measurement of bioaerosols Determination of moulds using filter sampling systems and culture-based analyses
    DIN ISO 16000-17:2010-06 Indoor air - Part 17: Detection and enumeration of moulds - Culture-based method (ISO 16000-17:2008)
    00/565216 DC : DRAFT DEC 2000 EN 14031 - WORKPLACE ATMOSPHERES - DETERMINATION OF AIRBORNE ENDOTOXIN
    09/30155944 DC : DRAFT MAY 2009 BS ISO 16000-18 - INDOOR AIR - PART 18: DETECTION AND ENUMERATION OF MOULDS - SAMPLING BY IMPACTION
    DIN EN ISO 16000-19:2014-12 Indoor air - Part 19: Sampling strategy for moulds (ISO 16000-19:2012)
    BS ISO 16000-18:2011 Indoor air Detection and enumeration of moulds. Sampling by impaction
    I.S. CEN/TR 15230:2005 WORKPLACE ATMOSPHERES - GUIDANCE FOR SAMPLING OF INHALABLE, THORACIC AND RESPIRABLE AEROSOL FRACTIONS
    ISO 16000-19:2012 Indoor air — Part 19: Sampling strategy for moulds
    I.S. EN 14042:2003 WORKPLACE ATMOSPHERES - GUIDE FOR THE APPLICATION AND USE OF PROCEDURES FOR THE ASSESSMENT OF EXPOSURE TO CHEMICAL AND BIOLOGICAL AGENTS
    VDI 4250 Blatt 2:2015-11 Bioaerosols and biological agents - Environmental health assessment of bioaerosols in ambient air - Risk assessment for aerosols containing Legionella
    VDI 4252 Blatt 2:2004-06 Measurement of airborne microorganisms and viruses in ambient air - Active sampling of bioaerosols - Separation of airborne mould on gelatine/polycarbonate filters
    VDI 4251-1 : 2007 COR 2 2016 MEASUREMENT OF AIRBORNE MICROORGANISMS AND VIRUSES IN AMBIENT AIR - PLANNING OF PLANT-RELATED AMBIENT AIR MEASUREMENTS - PLUME MEASUREMENT
    VDI 2083 Blatt 18:2012-01 Cleanroom technology - Biocontamination control
    VDI 4253 Blatt 2:2004-06 Measurement of airborne microorganisms and viruses in ambient air - Culture based method for the determination of the concentration of mould in air - Indirect method after sampling with gelatine/polycarbonate filters
    VDI 4255 Blatt 2:2009-12 Bioaerosols and biological agents - Emission sources and control measures in livestock operations - Overview
    VDI 4257 Blatt 1:2013-05 Bioaerosols and biological agents - Emission measurement - Planning and performing emission measurements
    VDI 4250 Blatt 3:2016-08 Bioaerosols and biological agents - Installation-specific measurement parameters and assessment values relevant to environmental health
    VDI 4258 Blatt 1:2017-03 Bioaerosols and biological agents - Generation of test bioaerosols - Basics and requirements for test bioaerosols
    VDI 4257 Blatt 2:2011-09 Bioaerosols and biological agents - Emission measurement - Sampling of bioaerosols and separation in liquids
    VDI 4253 Blatt 4:2013-02 Bioaerosols and biological agents - Determination of total cell count by fluorescence analysis after staining with DAPI
    VDI 3459 Blatt 4:2016-09 (Draft) Terminology of waste treatment and energy management - Biological treatment
    VDI 4255-1 : 2005 COR 2016 BIOAEROSOLS AND BIOLOGICAL AGENTS - SOURCES OF EMISSIONS AND CONTROL MEASURES - OVERVIEW
    VDI 4255 Blatt 3:2016-12 Bioaerosols and biological agents - Emission factors for poultry
    VDI 4250 Blatt 1:2014-08 Bioaerosols and biological agents - Risk assessment of source-related ambient air measurements in the scope of environmental health - Effects of bioaerosol pollution on human health
    VDI 4256 Blatt 1:2010-10 Bioaerosols and biological agents - Determination of performance characteristics - Culture-based counting methods
    VDI 4253 Blatt 3:2008-08 Measurement of airborne microorganisms and viruses in ambient air - Culture based method for the quantitative determination of bacteria in air - Method after separation in liquids
    VDI 4251 Blatt 2:2015-08 Measurement of airborne microorganisms and viruses in ambient air - Determination of area-specific background concentrations
    VDI 2083 Blatt 13.2:2009-01 Cleanroom technology - Quality, production and distribution of ultrapure water - Microelectronics and other technical applications
    VDI 4251 Blatt 4:2017-01 Measurement of airborne microorganisms and viruses in ambient air - Determination of existing load
    06/30146339 DC : 0 ISO 16000-16 - INDOOR AIR - PART 16: DETECTION AND ENUMERATION OF MOULDS - SAMPLING BY FILTRATION
    PD CEN/TS 16115-2:2016 Ambient air. Measurement of bioaerosols Planning and evaluation of plant-related plume measurements
    BS ISO 16000-17:2008 Indoor air Detection and enumeration of moulds. Culture-based method
    UNE-CEN/TS 16115-1:2013 Ambient air quality - Measurement of bioaerosols - Part 1: Determination of moulds using filter sampling systems and culture-based analyses
    CEN/TR 15230:2005 Workplace atmospheres - Guidance for sampling of inhalable, thoracic and respirable aerosol fractions
    UNI EN ISO 16000-19 : 2014 INDOOR AIR - PART 19: SAMPLING STRATEGY FOR MOULDS
    I.S. EN ISO 16000-19:2014 INDOOR AIR - PART 19: SAMPLING STRATEGY FOR MOULDS (ISO 16000-19:2012)
    PD CEN/TR 15230:2005 Workplace atmospheres. Guidance for sampling of inhalable, thoracic and respirable aerosol fractions
    DIN ISO 16000-20:2015-11 INDOOR AIR - PART 20: DETECTION AND ENUMERATION OF MOULDS - DETERMINATION OF TOTAL SPORE COUNT (ISO 16000-20:2014)
    I.S. EN 14031:2003 WORKPLACE ATMOSPHERES - DETERMINATION OF AIRBORNE ENDOTOXINS
    DIN EN 14583 E : 2004 WORKPLACE ATMOSPHERES - VOLUMETRIC BIOAEROSOL SAMPLING DEVICES - REQUIREMENTS AND TEST METHODS
    ISO 16000-17:2008 Indoor air — Part 17: Detection and enumeration of moulds — Culture-based method
    ISO 16000-18:2011 Indoor air Part 18: Detection and enumeration of moulds Sampling by impaction
    BS EN 14042:2003 Workplace atmospheres. Guide for the application and use of procedures for the assessment of exposure to chemical and biological agents
    ISO 16000-16:2008 Indoor air — Part 16: Detection and enumeration of moulds — Sampling by filtration
    EN ISO 16000-19:2014 Indoor air - Part 19: Sampling strategy for moulds (ISO 16000-19:2012)
    EN 14042:2003 Workplace atmospheres - Guide for the application and use of procedures for the assessment of exposure to chemical and biological agents
    DIN ISO 16000-18:2012-01 Indoor air - Part 18: Detection and enumeration of moulds - Sampling by impaction (ISO 16000-18:2011 + Cor.1:2011)
    DIN EN ISO 16000-19 E : 2014 INDOOR AIR - PART 19: SAMPLING STRATEGY FOR MOULDS (ISO 16000-19:2012)
    12/30261768 DC : 0 BS ISO 16000-21 - INDOOR AIR - PART 21: DETECTION AND ENUMERATION OF MOULDS - SAMPLING FROM MATERIALS
    BS EN ISO 16000-19:2014 Indoor air Sampling strategy for moulds
    DIN ISO 16000-19:2012-12 INDOOR AIR - PART 19: SAMPLING STRATEGY FOR MOULDS (ISO 16000-19:2012)
    DIN ISO 16000-16:2009-12 Indoor air - Part 16: Detection and enumeration of moulds - Sampling by filtration (ISO 16000-16:2008)
    S.R. CEN/TS 16115-1:2011 AMBIENT AIR QUALITY - MEASUREMENT OF BIOAEROSOLS - PART 1: DETERMINATION OF MOULDS USING FILTER SAMPLING SYSTEMS AND CULTURE-BASED ANALYSES
    S.R. CEN/TS 16115-2:2016 AMBIENT AIR - MEASUREMENT OF BIOAEROSOLS - PART 2: PLANNING AND EVALUATION OF PLANT-RELATED PLUME MEASUREMENTS
    BS EN 14583:2004 Workplace atmospheres. Volumetric bioaerosol sampling devices. Requirements and test methods
    DIN EN 14031:2003-06 Workplace atmospheres - Determination of airborne endotoxins
    DIN EN 14583:2004-12 Workplace atmospheres - Volumetric bioaerosol sampling devices - Requirements and test methods
    I.S. EN 14583:2004 WORKPLACE ATMOSPHERES - VOLUMETRIC BIOAEROSOL SAMPLING DEVICES - REQUIREMENTS AND TEST METHODS
    DIN EN 14042:2003-07 WORKPLACE ATMOSPHERES - GUIDE FOR THE APPLICATION AND USE OF PROCEDURES FOR THE ASSESSMENT OF EXPOSURE TO CHEMICAL AND BIOLOGICAL AGENTS
    EN 14583:2004 Workplace atmospheres - Volumetric bioaerosol sampling devices - Requirements and test methods

    Standards Referencing This Book - (Show below) - (Hide below)

    EN 12919:1999 Workplace atmospheres - Pumps for the sampling of chemical agents with a volume flow rate of over 5 l/min - Requirements and test methods
    ISO 7218:2007 Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
    EN 481:1993 Workplace atmospheres - Size fraction definitions for measurement of airborne particles
    EN 482:2012+A1:2015 Workplace exposure - General requirements for the performance of procedures for the measurement of chemical agents
    EN 1232:1997 Workplace atmospheres - Pumps for personal sampling of chemical agents - Requirements and test methods
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