1 Scope
2 Normative references
3 Terms and definitions
4 Safety precautions
5 Principle
6 Diluent, culture media and reagents
6.1 Basic materials
6.2 Diluent
7 Apparatus and glassware
8 Microbiological reference cultures
9 Sampling
10 Preparation of test material
10.1 Culturing and maintenance of host strains
10.2 Calibration of absorbance measurements for
counts of viable microorganisms
11 Procedure
11.1 Preparation of inoculum cultures
11.2 Standard procedure
11.3 Method for samples with high bacterial
background flora
11.4 Samples with low phage counts
11.5 Presence/absence test
11.6 Quality assurance
12 Expression of results
12.1 Plaque count procedures (11.2 to 11.4)
12.2 Presence/absence test (11.5)
13 Test report
Annex A (normative) Culture media, reagents and
diluent
Annex B (informative) General description of
somatic bacteriophages
Annex C (informative) Culturing of bacteriophage
PhiX174
Bibliography