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CEN
EN ISO 16931:2009

EN ISO 16931:2009

Current

Current

The latest, up-to-date edition.

Animal and vegetable fats and oils - Determination of polymerized triacylglycerols by high-performance size-exclusion chromatography (HPSEC) (ISO 16931:2009)

Published date

15-04-2009

Publisher

Comite Europeen de Normalisation

Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus
7 Sampling
8 Preparation of test sample
9 Procedure
10 Expression of results
11 Precision
12 Test report
Annex A (informative) - Chromatograms
Annex B (informative) - Results of interlaboratory tests
Bibliography

ISO 16931:2009 specifies a method using high-performance size-exclusion chromatography (HPSEC) to determine the contents, as mass fractions, of polymerized triacylglycerols (PTAGs) in oils and fats which contain at least 3 % (from peak areas) of these polymers. PTAGs (strictly speaking dimeric and oligomeric triacylglycerols) are formed during the heating of fats and oils, and thus, the method serves to assess the thermal deterioration of frying fats after use.This method is applicable to frying fats and fats and oils that have been thermally treated, provided that the content of PTAGs is at least 3 %. It can also be applied to the determination of polymers in fats for animal feedstuffs, although in this case, the extraction method used can have an influence on the result.

Committee
CEN/TC 307
DocumentType
Standard
PublisherName
Comite Europeen de Normalisation
Status
Current

ISO 6492:1999 Animal feeding stuffs — Determination of fat content
ISO 5725-1:1994 Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions
ISO 5555:2001 Animal and vegetable fats and oils — Sampling
ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
ISO 661:2003 Animal and vegetable fats and oils Preparation of test sample

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