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CEN
EN ISO 21871:2006

EN ISO 21871:2006

Current

Current

The latest, up-to-date edition.

Microbiology of food and animal feeding stuffs - Horizontal method for the determination of low numbers of presumptive Bacillus cereus - Most probable number technique and detection method (ISO 21871:2006)

Published date

15-01-2006

Publisher

Comite Europeen de Normalisation

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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
   4.1 Enumeration method
   4.2 Detection method
5 Diluent, culture media and reagents
   5.1 General
   5.2 Diluent
   5.3 Liquid selective enrichment medium:
       Tryptone soya polymyxin broth (TSPB)
   5.4 Solid selective medium: Polymyxin pyruvate
       egg yolk mannitol bromothymol blue agar (PEMBA)
   5.5 Solid selective medium: Mannitol egg yolk
       polymyxin agar (MYP)
   5.6 Staining solutions for microscopic identification
   5.7 Sheep blood agar
6 Apparatus and glassware
7 Sampling
8 Preparation of test sample
9 Procedure
   9.1 Enumeration method(MPN)
   9.2 Detection method
10 Calculation and expression of results
   10.1 Enumeration method for the determination of the
        most probable number (MPN)
   10.2 Detection method
11 Test report
Annex A (normative) Diagram of enumeration procedure
Bibliography

ISO 21871:2006 specifies a horizontal method for the detection or the enumeration of low numbers of viable presumptive Bacillus cereus by means of the most probable number technique. ISO 21871:2006 is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.

Committee
CEN/TC 463
DocumentType
Standard
PublisherName
Comite Europeen de Normalisation
Status
Current

ISO/TS 11133-1:2009 Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 1: General guidelines on quality assurance for the preparation of culture media in the laboratory
ISO 7218:2007 Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations
ISO/TS 11133-2:2003 Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 2: Practical guidelines on performance testing of culture media
ISO 8261:2001 Milk and milk products General guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination

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