I.S. EN ISO 15216-1:2017&A1:2021
Current
The latest, up-to-date edition.
Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using realtime RT-PCR - Part 1: Method for quantification (ISO 15216-1:2017)
Hardcopy , PDF
English
27-05-2021
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Equipment and consumables
7 Sampling
8 Procedure
9 Interpretation of results
10 Expression of results
11 Precision
12 Test report
Annex A (normative) Diagram of procedure
Annex B (normative) Composition and preparation of reagents and buffers
Annex C (informative) Real-time RT-PCR mastermixes and cycling parameters
Annex D (informative) Real-time RT-PCR primers and hydrolysis probes for the detection of HAV, norovirus GI and GII and mengo virus (process control)
Annex E (informative) Growth of mengo virus strain MC0 for use as a process control
Annex F (informative) RNA extraction using the NucliSENS® system
Annex G (informative) Generation of dsDNA control stocks
Annex H (informative) Generation of EC RNA stocks
Annex I (informative) Typical optical plate layout
Annex J (informative) Method validation studies and performance characteristics
Bibliography
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