I.S. EN ISO 15216-1:2017
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
MICROBIOLOGY OF THE FOOD CHAIN - HORIZONTAL METHOD FOR DETERMINATION OF HEPATITIS A VIRUS AND NOROVIRUS USING REAL-TIME RT-PCR - PART 1: METHOD FOR QUANTIFICATION (ISO 15216-1:2017)
Hardcopy , PDF
31-05-2021
English
01-01-2017
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Only cited Standards give presumption of conformance to New Approach Directives/Regulations.
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National Foreword
European foreword
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Equipment and consumables
7 Sampling
8 Procedure
9 Interpretation of results
10 Expression of results
11 Precision
12 Test report
Annex A (normative) - Diagram of procedure
Annex B (normative) - Composition and preparation of reagents
and buffers
Annex C (informative) - Real-time RT-PCR mastermixes and
cycling parameters
Annex D (informative) - Real-time RT-PCR primers and
hydrolysis probes for the detection of HAV, norovirus
GI and GII and mengo virus (process control)
Annex E (informative) - Growth of mengo virus strain MC[0]
for use as a process control
Annex F (informative) - RNA extraction using the NucliSENS[R]
system
Annex G (informative) - Generation of dsDNA control stocks
Annex H (informative) - Generation of EC RNA stocks
Annex I (informative) - Typical optical plate layout
Annex J (informative) - Method validation studies and
performance characteristics
Bibliography
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