I.S. EN ISO 17601:2018
Current
The latest, up-to-date edition.
SOIL QUALITY - ESTIMATION OF ABUNDANCE OF SELECTED MICROBIAL GENE SEQUENCES BY QUANTITATIVE PCR FROM DNA DIRECTLY EXTRACTED FROM SOIL (ISO 17601:2016)
Hardcopy , PDF
English
01-01-2018
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National Foreword
European foreword
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Test materials
6 Apparatus
7 Procedure
8 Examination of the critical steps of the [q]PCR assay
9 Expression of the results of the [q]PCR assay
10 International ring test
11 Test report
Annex A (informative) - Description of principal steps of
TaqMan[R] [q]PCR assay
Annex B (informative) - International ring-test for evaluating
[q]PCR to quantify the abundance of selected microbial
gene sequences from DNA directly extracted from soil
Bibliography
Defines the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups.
| DocumentType |
Standard
|
| Pages |
48
|
| PublisherName |
National Standards Authority of Ireland
|
| Status |
Current
|
| Standards | Relationship |
| ISO 17601:2016 | Identical |
| EN ISO 17601:2018 | Identical |
| ISO 10381-6:2009 | Soil quality Sampling Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory |
| ISO 11063:2012 | Soil quality Method to directly extract DNA from soil samples |
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