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ISO 11063:2012

Withdrawn

Withdrawn

A Withdrawn Standard is one, which is removed from sale, and its unique number can no longer be used. The Standard can be withdrawn and not replaced, or it can be withdrawn and replaced by a Standard with a different number.

View Superseded by

Soil quality Method to directly extract DNA from soil samples

Available format(s)

Hardcopy , PDF , PDF 3 Users , PDF 5 Users , PDF 9 Users

Withdrawn date

22-10-2020

Superseded by

ISO 11063:2020

Language(s)

French, English

Published date

06-02-2012

€60.00
Excluding VAT

This International Standard specifies a method for direct extraction of DNA from soil samples to analyse the

global structure and the abundance of soil bacterial communities using PCR-based technologies. This method

is mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich in

organic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals.

The direct extraction of DNA from soil samples provides unique insight into the richness and structure of

microbial communities which are key parameters to estimate the biodiversity of soil microbiota. Molecular

approaches based on PCR (polymerase chain reaction) amplification of soil DNA constitute a promising

domain and can contribute in the near future to the development of routine tools to monitor the microbiota of

soil environments.

Users of the method ought to be aware that although soil submitted to the DNA extraction procedure is sieved

thoroughly (2 mm mesh, procedure described in 5.1), plant residues can still remain in soil samples and, as a

result, traces of plant DNA can contaminate the soil DNA extract.

DevelopmentNote
Supersedes ISO/DIS 11063. (02/2012)
DocumentType
Standard
Pages
24
PublisherName
International Organization for Standardization
Status
Withdrawn
SupersededBy

17/30333759 DC : 0 BS ISO 18400-206 - SOIL QUALITY - SAMPLING - PART 206: GUIDANCE ON THE COLLECTION, HANDLING AND STORAGE OF SOIL FOR THE ASSESSMENT OF BIOLOGICAL FUNCTIONAL AND STRUCTURAL ENDPOINTS IN THE LABORATORY
13/30253328 DC : 0 BS ISO 17601 - SOIL QUALITY - ESTIMATION OF ABUNDANCE OF SELECTED MICROBIAL GENE SEQUENCES BY QUANTITATIVE REALTIME PCR FROM DNA DIRECTLY EXTRACTED FROM SOIL
I.S. EN ISO 17601:2018 SOIL QUALITY - ESTIMATION OF ABUNDANCE OF SELECTED MICROBIAL GENE SEQUENCES BY QUANTITATIVE PCR FROM DNA DIRECTLY EXTRACTED FROM SOIL (ISO 17601:2016)
ISO 17601:2016 Soil quality — Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil
BS ISO 17601 : 2016 COR 2018 SOIL QUALITY - ESTIMATION OF ABUNDANCE OF SELECTED MICROBIAL GENE SEQUENCES BY QUANTITATIVE PCR FROM DNA DIRECTLY EXTRACTED FROM SOIL
ISO 19204:2017 Soil quality — Procedure for site-specific ecological risk assessment of soil contamination (soil quality TRIAD approach)
EN ISO 17601:2018 Soil quality - Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil (ISO 17601:2016)
17/30352199 DC : 0 BS ISO 15799 - SOIL QUALITY - GUIDANCE ON THE ECOTOXICOLOGICAL CHARACTERIZATION OF SOILS AND SOIL MATERIALS
BS ISO 19204:2017 Soil quality. Procedure for site-specific ecological risk assessment of soil contamination (soil quality TRIAD approach)
DIN ISO 19204:2015-09 (Draft) SOIL QUALITY - PROCEDURE FOR SITE-SPECIFIC ECOLOGICAL RISK ASSESSMENT OF SOIL CONTAMINATION (SOIL QUALITY TRIAD APPROACH) (ISO 19204:2017)

ISO 10381-6:2009 Soil quality Sampling Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory

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