ISO 12966-3:2016
Current
The latest, up-to-date edition.
Animal and vegetable fats and oils Gas chromatography of fatty acid methyl esters Part 3: Preparation of methyl esters using trimethylsulfonium hydroxide (TMSH)
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French, English
12-05-2016
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ISO 12966-3:2016 specifies a rapid base-catalysed transesterification method for fats and oils with trimethylsulfonium hydroxide (TMSH) to prepare fatty acid methyl esters. The method is exclusively applicable to the preparation of methyl esters of fats and oils for gas liquid chromatographic (GLC) analysis. It is applicable to all fats and oils, but excluding those coming from milk and milk products. Isomerization of unsaturated fatty acids only occurs to a minor extent and isomerized fatty acids are only present at the determination limit. As isomerization takes place, the procedure is not recommended for conjugated linoleic acid (CLA).
Only about 70 % to 80 % of the free fatty acids are esterified. In the case of conjugated cyclopropyl and cyclopropenyl fatty acids, side reactions may occur, but these do not interfere with the determination of the fatty acids.
| DevelopmentNote |
Supersedes ISO/DIS 12966-3. (05/2016)
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| DocumentType |
Standard
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| Pages |
4
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| PublisherName |
International Organization for Standardization
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| Status |
Current
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| Supersedes |
| Standards | Relationship |
| NF EN ISO 12966-3 : 2016 | Identical |
| NBN EN ISO 12966-3 : 2016 | Identical |
| NEN EN ISO 12966-3 : 2016 | Identical |
| NS EN ISO 12966-3 : 2009 | Identical |
| I.S. EN ISO 12966-3:2016 | Identical |
| PN EN ISO 12966-3 : 2016 | Identical |
| SN EN ISO 12966-3:2016 | Identical |
| UNI EN ISO 12966-3 : 2010 | Identical |
| SS-EN ISO 12966-3:2016 | Identical |
| UNI EN ISO 12966-3:2016 | Identical |
| UNE-EN ISO 12966-3:2016 | Identical |
| BS EN ISO 12966-3:2016 | Identical |
| EN ISO 12966-3:2016 | Identical |
| DIN EN ISO 12966-3:2016-11 | Identical |
| 13/30281563 DC : 0 | BS EN ISO 12966-1 - OILS - GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS - PART 1: GUIDELINES ON GAS CHROMATOGRAPHY |
| 14/30286016 DC : 0 | BS EN ISO 12966-4 - ANIMAL AND VEGETABLE FATS AND OILS - DETERMINATION OF METHYL ESTERS OF FATTY ACIDS - PART 4: CAPILLARY GAS CHROMATOGRAPHIC METHOD |
| DIN EN ISO 12966-1:2015-10 | ANIMAL AND VEGETABLE FATS AND OILS - GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS - PART 1: GUIDELINES ON MODERN GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS (ISO 12966-1:2014) |
| BS EN ISO 12966-4:2015 | Animal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters Determination by capillary gas chromatography |
| DIN EN ISO 3657:2013-12 | ANIMAL AND VEGETABLE FATS AND OILS - DETERMINATION OF SAPONIFICATION VALUE (ISO 3657:2013) |
| ISO 3657:2013 | Animal and vegetable fats and oils Determination of saponification value |
| UNI EN ISO 3657 : 2013 | ANIMAL AND VEGETABLE FATS AND OILS - DETERMINATION OF SAPONIFICATION VALUE |
| BS EN ISO 12966-1:2014 | Animal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters Guidelines on modern gas chromatography of fatty acid methyl esters |
| I.S. EN ISO 12966-4:2015 | ANIMAL AND VEGETABLE FATS AND OILS - GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS - PART 4: DETERMINATION BY CAPILLARY GAS CHROMATOGRAPHY (ISO 12966-4:2015) |
| I.S. EN ISO 12966-1:2014 | ANIMAL AND VEGETABLE FATS AND OILS - GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS - PART 1: GUIDELINES ON MODERN GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS (ISO 12966-1:2014) |
| BS EN ISO 3657:2013 | Animal and vegetable fats and oils. Determination of saponification value |
| I.S. EN ISO 3657:2013 | ANIMAL AND VEGETABLE FATS AND OILS - DETERMINATION OF SAPONIFICATION VALUE (ISO 3657:2013) |
| UNE-EN ISO 3657:2013 | Animal and vegetable fats and oils - Determination of saponification value (ISO 3657:2013) |
| EN ISO 3657:2013 | Animal and vegetable fats and oils - Determination of saponification value (ISO 3657:2013) |
| ISO 12966-4:2015 | Animal and vegetable fats and oils Gas chromatography of fatty acid methyl esters Part 4: Determination by capillary gas chromatography |
| UNE-EN ISO 12966-1:2015 | Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 1: Guidelines on modern gas chromatography of fatty acid methyl esters (ISO 12966-1:2014) |
| EN ISO 12966-4:2015 | Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 4: Determination by capillary gas chromatography (ISO 12966-4:2015) |
| DIN EN ISO 12966-4:2015-11 | ANIMAL AND VEGETABLE FATS AND OILS - GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS - PART 4: DETERMINATION BY CAPILLARY GAS CHROMATOGRAPHY (ISO 12966-4:2015) |
| ISO 5555:2001 | Animal and vegetable fats and oils — Sampling |
| ISO 1042:1998 | Laboratory glassware — One-mark volumetric flasks |
| ISO 835:2007 | Laboratory glassware — Graduated pipettes |
| ISO 661:2003 | Animal and vegetable fats and oils — Preparation of test sample |
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