ISO 21569:2005
Current
Current
The latest, up-to-date edition.
Foodstuffs — Methods of analysis for the detection of genetically modified organisms and derived products — Qualitative nucleic acid based methods
Amended by
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Published date
20-06-2005
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ISO 21569:2005 describes the procedure to qualitatively detect genetically modified organisms (GMOs) and derived products by analysing the nucleic acids extracted from the sample under study. The main focus is on polymerase chain reaction (PCR) based amplification methods.
It gives general requirements for the specific detection and identification of target nucleic acid sequences (DNA) and for the confirmation of the identity of the amplified DNA sequence.
Guidelines, minimum requirements and performance criteria laid down in ISO 21569:2005 are intended to ensure that comparable, accurate and reproducible results are obtained in different laboratories.
ISO 21569:2005 has been established for food matrices, but could also be applied to other matrices (e.g. feed and plant samples from the environment).
Specific examples of methods are provided in Annexes A to D.
| Committee |
ISO/TC 34/SC 16
|
| DevelopmentNote |
Supersedes ISO/DIS 21569 (06/2005)
|
| DocumentType |
Standard
|
| Pages |
69
|
| ProductNote |
THIS STANDARD ALSO REFERS TO ISO 21568,
|
| PublisherName |
International Organization for Standardization
|
| Status |
Current
|
| Standards | Relationship |
| UNE-EN ISO 21569:2006 | Identical |
| BIS IS/ISO 21569 : 2005 AMD 1 2015 | Identical |
| BS EN ISO 21569 : 2005 | Identical |
| EN ISO 21569 : 2005 AMD 1 2013 | Identical |
| DIN EN ISO 21569:2013-08 | Identical |
| NF EN ISO 21569 : 2006 AMD 1 2013 | Identical |
| NBN EN ISO 21569 : 2005 AMD 1 2013 | Identical |
| NEN EN ISO 21569 : 2005 AMD 1 2013 | Identical |
| NS EN ISO 21569 : 2005 AMD 1 2013 | Identical |
| I.S. EN ISO 21569:2005 | Identical |
| PN EN ISO 21569 : 2007 AMD 1 2013 | Identical |
| SN EN ISO 21569 : 2005 AMD 1 2013 | Identical |
| UNI EN ISO 21569 : 2013 | Identical |
| NF EN ISO 21569 April 2006 | Identical |
| NEN-EN-ISO 21569 : 2005 | Identical |
| PN-EN ISO 21569 : 2007 | Identical |
| SS-EN ISO 21569 : 2006 | Identical |
| SN EN ISO 21569 : 2005 | Identical |
| UNE-EN ISO 21569:2005 | Identical |
| BS EN ISO 21569:2005+A1:2013 | Identical |
| PD CEN/TS 16707:2014 | Foodstuffs. Methods of analysis for the detection of genetically modified organisms and derived products. Polymerase chain reaction (PCR) based screening strategies |
| S.R. CEN/TS 16707:2014 | FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - POLYMERASE CHAIN REACTION (PCR) BASED SCREENING STRATEGIES |
| ISO/TS 21569-5:2016 | Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 5: Real-time PCR based screening method for the detection of the FMV promoter (P-FMV) DNA sequence |
| ISO/TS 17822-1:2014 | In vitro diagnostic test systems Qualitative nucleic acid-based in vitro examination procedures for detection and identification of microbial pathogens Part 1: General requirements, terms and definitions |
| UNE-EN ISO 21570:2008 | Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods (ISO 21570:2005) |
| PD ISO/TS 17822-1:2014 | <i>In</i> <i>vitro</i> diagnostic test systems. Qualitative nucleic acid-based <i>in</i> <i>vitro</i> examination procedures for detection and identification of microbial pathogens General requirements, terms and definitions |
| PD ISO/TS 21569-3:2015 | Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products Construct-specific real-time PCR method for detection of P35S-pat-sequence for screening genetically modified organisms |
| DD CEN/TS 15568:2006 | Foodstuffs. Methods of analysis for the detection of genetically modified organisms and derived products. Sampling strategies |
| ISO/TS 21569-3:2015 | Horizontal methods for molecular biomarker analysis Methods of analysis for the detection of genetically modified organisms and derived products Part 3: Construct-specific real-time PCR method for detection of P35S-pat-sequence for screening genetically modified organisms |
| UNI EN ISO 21570 : 2013 | FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - QUANTITATIVE NUCLEIC ACID BASED METHODS |
| VDI 4330 Blatt 7:2006-12 | Monitoring the effects of genetically modified organisms (GMO) - Qualitative methods for the detection of genetically engineered nucleic acids in the environment |
| ISO/TS 21569-6:2016 | Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 6: Real-time PCR based screening methods for the detection of cry1Ab/Ac and Pubi-cry DNA sequences |
| DD ISO/TS 21098:2005 | Foodstuffs. Nucleic acid based methods of analysis of genetically modified organisms and derived products. Information to be supplied and procedure for the addition of methods to ISO 21569, ISO 21570 and ISO 21571 |
| PD ISO/TS 21569-2:2012 | Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products Construct-specific real-time PCR method for detection of event FP967 in linseed and linseed products |
| ISO/TS 21569-4:2016 | Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 4: Real-time PCR based screening methods for the detection of the P-nos and P-nos-nptII DNA sequences |
| DIN EN ISO 21571:2013-08 | FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - NUCLEIC ACID EXTRACTION (ISO 21571:2005 + AMD 1:2013) |
| UNI EN ISO 21571 : 2013 | FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - NUCLEIC ACID EXTRACTION |
| CEN/TS 16707:2014 | Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Polymerase chain reaction (PCR) based screening strategies |
| PD ISO/TS 21569-4:2016 | Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products Real-time PCR based screening methods for the detection of the <i>P</i>-<i>nos</i> and <i>P</i>-<i>nos</i>-<i>nptII</i> DNA sequences |
| PD ISO/TS 21569-5:2016 | Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products Real-time PCR based screening method for the detection of the FMV promoter (P-FMV) DNA sequence |
| ISO/TS 21569-2:2012 | Horizontal methods for molecular biomarker analysis Methods of analysis for the detection of genetically modified organisms and derived products Part 2: Construct-specific real-time PCR method for detection of event FP967 in linseed and linseed products |
| I.S. CEN TS 15568:2006 | FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - SAMPLING STRATEGIES |
| ISO 21570:2005 | Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Quantitative nucleic acid based methods |
| BS EN ISO 21570 : 2005 | FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - QUANTITATIVE NUCLEIC ACID BASED METHODS |
| ISO 21571:2005 | Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Nucleic acid extraction |
| DIN EN ISO 21570:2013-08 | Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods (ISO 21570:2005 + Cor 1:2006 + Amd 1:2013) |
| UNI CEN/TS 16707 : 2014 | FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - POLYMERASE CHAIN REACTION (PCR) BASED SCREENING STRATEGIES |
| ISO/TS 21098:2005 | Foodstuffs Nucleic acid based methods of analysis of genetically modified organisms and derived products Information to be supplied and procedure for the addition of methods to ISO 21569, ISO 21570 or ISO 21571 |
| BS EN ISO 21571 : 2005 | FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - NUCLEIC ACID EXTRACTION |
| CEN/TS 15568:2006 | Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Sampling strategies |
| ISO/IEC 17025:2005 | General requirements for the competence of testing and calibration laboratories |
| ISO 5725-1:1994 | Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions |
| ISO 24276:2006 | Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products General requirements and definitions |
| ISO 21571:2005 | Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Nucleic acid extraction |
| ISO 5725-2:1994 | Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method |
| ISO 21570:2005 | Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Quantitative nucleic acid based methods |
| ISO/TS 21098:2005 | Foodstuffs Nucleic acid based methods of analysis of genetically modified organisms and derived products Information to be supplied and procedure for the addition of methods to ISO 21569, ISO 21570 or ISO 21571 |
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