• ISO 9308-1:2014

    Current The latest, up-to-date edition.

    Water quality Enumeration of Escherichia coli and coliform bacteria Part 1: Membrane filtration method for waters with low bacterial background flora

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    Published date:  16-09-2014

    Publisher:  International Organization for Standardization

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    ISO 9308-1:2014 specifies a method for the enumeration of Escherichia coli (E. coli) and coliform bacteria. The method is based on membrane filtration, subsequent culture on a chromogenic coliform agar medium, and calculation of the number of target organisms in the sample. Due to the low selectivity of the differential agar medium, background growth can interfere with the reliable enumeration of E. coli and coliform bacteria, for example, in surface waters or shallow well waters. This method is not suitable for these types of water.

    ISO 9308-1:2014 is especially suitable for waters with low bacterial numbers that will cause less than 100 total colonies on chromogenic coliform agar (CCA). These may be drinking water, disinfected pool water, or finished water from drinking water treatment plants.

    Some strains of E. coli which are -D-glucuronidase negative, such as Escherichia coli O157, will not be detected as E. coli. As they are -D-galactosidase positive, they will appear as coliform bacteria on this chromogenic agar.

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    Development Note Supersedes ISO/DIS 9308-1. (09/2014)
    Document Type Standard
    Publisher International Organization for Standardization
    Status Current
    Supersedes

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    VDI 4066 Blatt 4:2016-04 Hygiene requirements for the production and filling of dairy products without recontamination
    BS 6068-1.7:1997 Water quality. Glossary An additional 51 terms
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    Standards Referencing This Book - (Show below) - (Hide below)

    ISO 7704:1985 Water quality — Evaluation of membrane filters used for microbiological analyses
    ISO 7218:2007 Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
    ISO 19458:2006 Water quality Sampling for microbiological analysis
    ISO 8199:2005 Water quality General guidance on the enumeration of micro-organisms by culture
    ISO 11133:2014 Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media
    ISO 3696:1987 Water for analytical laboratory use — Specification and test methods
    CFR 40(PTS136-149) : JUL 2017 PROTECTION OF ENVIRONMENT - ENVIRONMENTAL PROTECTION AGENCY
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