NEN EN ISO 10705-2 : 2001

1 Scope
2 Normative references
3 Terms and definitions
4 Safety precautions
5 Principle
6 Diluent, culture media and reagents
   6.1 Basic materials
   6.2 Diluent
7 Apparatus and glassware
8 Microbiological reference cultures
9 Sampling
10 Preparation of test material
   10.1 Culturing and maintenance of host strains
   10.2 Calibration of absorbance measurements for
        counts of viable microorganisms
11 Procedure
   11.1 Preparation of inoculum cultures
   11.2 Standard procedure
   11.3 Method for samples with high bacterial
        background flora
   11.4 Samples with low phage counts
   11.5 Presence/absence test
   11.6 Quality assurance
12 Expression of results
   12.1 Plaque count procedures (11.2 to 11.4)
   12.2 Presence/absence test (11.5)
13 Test report
Annex A (normative) Culture media, reagents and
        diluent
Annex B (informative) General description of
        somatic bacteriophages
Annex C (informative) Culturing of bacteriaophage
        X174
Bibliography

Describes a method for the detection and enumeration of somatic coliphages by incubating the sample with an appropriate host strain. Applicable to all kinds of water, sediments and sludge extracts, where necessary after dilution.