UNE-EN ISO 17601:2018
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
View Superseded by
Soil quality - Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil (ISO 17601:2016) (Endorsed by Asociación Española de Normalización in April of 2018.)
Hardcopy , PDF
English
01-04-2018
01-12-2025
This International Standard specifies the crucial steps of a quantitative real-time polymerase chainreaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNAextract which provides an estimation of selected microbial groups.It is noteworthy that the number of genes is not necessarily directly linked to the number of organismsthat are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copiesin different bacterial phyla. Therefore, the number of 16S rRNA sequences quantified from soil DNAextracts does not give an exact estimate of the number of soil bacteria. Furthermore, the number ofsequences is not necessarily linked to living microorganisms and can comprise sequences amplifiedfrom dead microorganisms.
| Committee |
CTN 77/SC 3
|
| DocumentType |
Standard
|
| Pages |
41
|
| PublisherName |
Asociación Española de Normalización
|
| Status |
Superseded
|
| SupersededBy |
| Standards | Relationship |
| DIN EN ISO 17601:2018-08 | Equivalent |
| BS EN ISO 17601:2018 | Equivalent |
| I.S. EN ISO 17601:2018 | Equivalent |
| ISO 17601:2016 | Identical |
| EN ISO 17601:2018 | Identical |
| BS 3486-1:1962 | Identical |
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