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ASTM D 6974 : 2020

Current

Current

The latest, up-to-date edition.

Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

01-05-2020

€56.53
Excluding VAT

Committee
D 02
DocumentType
Standard Practice
Pages
6
PublisherName
American Society for Testing and Materials
Status
Current
Supersedes

1.1This practice covers a membrane filter (MF) procedure for the detection and enumeration of Heterotrophic bacteria (HPC) and fungi in liquid fuels with kinematic viscosities 24 mm2 · s-1 at ambient temperature.

1.2This quantitative practice is drawn largely from IP Method 385 and Test Method D5259.

1.3This test may be performed either in the field or in the laboratory.

1.4The ability of individual microbes to form colonies on specific growth media depends on the taxonomy and physiological state of the microbes to be enumerated, the chemistry of the growth medium, and incubation conditions. Consequently, test results should not be interpreted as absolute values. Rather they should be used as part of a diagnostic or condition monitoring effort that includes other test parameters, in accordance with Guide D6469.

1.5This practice offers alternative options for delivering fuel sample microbes to the filter membrane, volumes or dilutions filtered, growth media used to cultivate fuel-borne microbes, and incubation temperatures. This flexibility is offered to facilitate diagnostic efforts. When this practice is used as part of a condition monitoring program, a single procedure should be used consistently.

1.6The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.7This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.8This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

ASTM D 8070 : 2019 : REV A Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay
ASTM D 7847 : 2017 Standard Guide for Interlaboratory Studies for Microbiological Test Methods
ASTM D 6469 : 2017 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
ASTM E 3152 : 2018 Standard Guide for Standard Test Methods and Practices Available for Determining Antifungal Activity on Natural or Synthetic Substrates Treated with Antimicrobial Agents
ASTM E 2800 : 2011 : R2017 Standard Practice for Characterization of <emph type="ital">Bacillus</emph> Spore Suspensions for Reference Materials
ASTM D 5465 : 2016 Standard Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
ASTM D 7464 : 2019 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing

ASTM F 1094 : 1987 : R2020 Standard Test Methods for Microbiological Monitoring of Water Used for Processing Electron and Microelectronic Devices by Direct Pressure Tap Sampling Valve and by the Presterilized Plastic Bag Method (Withdrawn 2023)
ASTM D 7464 : 2020 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
ASTM D 4175 : 2020 : REV B Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 6469 : 2020 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
ASTM D 7463 : 2021 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water
ASTM D 1129 : 2013 : R2020 Standard Terminology Relating to Water
ASTM D 1129 : 2013 : R2020 : EDT 2 Standard Terminology Relating to Water
ASTM D 4175 : 2022 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 4175 : 2024 : EDT 1 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 6426 : 2022 Standard Test Method for Determining Filterability of Middle Distillate Fuel Oils
ASTM D 5259 : 2024 Standard Test Method for Isolation and Enumeration of Enterococci from Water by the Membrane Filter Procedure
ASTM D 4175 : 2001 : EDT 1 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM D 4175 : 2020 : REV B : EDT 1 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 4175 : 2022 : REV A Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 4175 : 2002 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM D 1129 : 2013 : R2020 : EDT 1 Standard Terminology Relating to Water
ASTM D 4175 : 2023 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 4175 : 2022 : REV A : EDT 1 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 7463 : 2018 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water
ASTM D 6426 : 2013 Standard Test Method for Determining Filterability of Middle Distillate Fuel Oils
ASTM D 6469 : 2024 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
ASTM D 6426 : 2001 Standard Test Method for Determining Filterability of Distillate Fuel Oils
ASTM D 4175 : 2023 : EDT 1 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 4175 : 2023 : REV A Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 4175 : 2022 : EDT 1 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 6426 : 2018 Standard Test Method for Determining Filterability of Middle Distillate Fuel Oils
ASTM D 4175 : 2024 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 5259 : 2019 Standard Test Method for Isolation and Enumeration of Enterococci from Water by the Membrane Filter Procedure

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