ASTM D 6974 : 2020
Current
The latest, up-to-date edition.
Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
Hardcopy , PDF
English
01-05-2020
Committee |
D 02
|
DocumentType |
Standard Practice
|
Pages |
6
|
PublisherName |
American Society for Testing and Materials
|
Status |
Current
|
Supersedes |
1.1This practice covers a membrane filter (MF) procedure for the detection and enumeration of Heterotrophic bacteria (HPC) and fungi in liquid fuels with kinematic viscosities ≤24 mm2 · s-1 at ambient temperature.
1.2This quantitative practice is drawn largely from IP Method 385 and Test Method D5259.
1.3This test may be performed either in the field or in the laboratory.
1.4The ability of individual microbes to form colonies on specific growth media depends on the taxonomy and physiological state of the microbes to be enumerated, the chemistry of the growth medium, and incubation conditions. Consequently, test results should not be interpreted as absolute values. Rather they should be used as part of a diagnostic or condition monitoring effort that includes other test parameters, in accordance with Guide D6469.
1.5This practice offers alternative options for delivering fuel sample microbes to the filter membrane, volumes or dilutions filtered, growth media used to cultivate fuel-borne microbes, and incubation temperatures. This flexibility is offered to facilitate diagnostic efforts. When this practice is used as part of a condition monitoring program, a single procedure should be used consistently.
1.6The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.7This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.
1.8This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
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ASTM D 6469 : 2017 | Standard Guide for Microbial Contamination in Fuels and Fuel Systems |
ASTM E 3152 : 2018 | Standard Guide for Standard Test Methods and Practices Available for Determining Antifungal Activity on Natural or Synthetic Substrates Treated with Antimicrobial Agents |
ASTM E 2800 : 2011 : R2017 | Standard Practice for Characterization of <emph type="ital">Bacillus</emph> Spore Suspensions for Reference Materials |
ASTM D 5465 : 2016 | Standard Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods |
ASTM D 7464 : 2019 | Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing |
ASTM F 1094 : 1987 : R2020 | Standard Test Methods for Microbiological Monitoring of Water Used for Processing Electron and Microelectronic Devices by Direct Pressure Tap Sampling Valve and by the Presterilized Plastic Bag Method (Withdrawn 2023) |
ASTM D 7464 : 2020 | Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing |
ASTM D 4175 : 2020 : REV B | Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants |
ASTM D 6469 : 2020 | Standard Guide for Microbial Contamination in Fuels and Fuel Systems |
ASTM D 7463 : 2021 | Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water |
ASTM D 1129 : 2013 : R2020 | Standard Terminology Relating to Water |
ASTM D 1129 : 2013 : R2020 : EDT 2 | Standard Terminology Relating to Water |
ASTM D 4175 : 2022 | Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants |
ASTM D 4175 : 2024 : EDT 1 | Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants |
ASTM D 6426 : 2022 | Standard Test Method for Determining Filterability of Middle Distillate Fuel Oils |
ASTM D 5259 : 2024 | Standard Test Method for Isolation and Enumeration of Enterococci from Water by the Membrane Filter Procedure |
ASTM D 4175 : 2001 : EDT 1 | Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants |
ASTM D 4175 : 2020 : REV B : EDT 1 | Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants |
ASTM D 4175 : 2022 : REV A | Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants |
ASTM D 4175 : 2002 | Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants |
ASTM D 1129 : 2013 : R2020 : EDT 1 | Standard Terminology Relating to Water |
ASTM D 4175 : 2023 | Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants |
ASTM D 4175 : 2022 : REV A : EDT 1 | Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants |
ASTM D 7463 : 2018 | Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water |
ASTM D 6426 : 2013 | Standard Test Method for Determining Filterability of Middle Distillate Fuel Oils |
ASTM D 6469 : 2024 | Standard Guide for Microbial Contamination in Fuels and Fuel Systems |
ASTM D 6426 : 2001 | Standard Test Method for Determining Filterability of Distillate Fuel Oils |
ASTM D 4175 : 2023 : EDT 1 | Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants |
ASTM D 4175 : 2023 : REV A | Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants |
ASTM D 4175 : 2022 : EDT 1 | Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants |
ASTM D 6426 : 2018 | Standard Test Method for Determining Filterability of Middle Distillate Fuel Oils |
ASTM D 4175 : 2024 | Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants |
ASTM D 5259 : 2019 | Standard Test Method for Isolation and Enumeration of Enterococci from Water by the Membrane Filter Procedure |
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