BS EN 16187:2015
Current
The latest, up-to-date edition.
Foodstuffs. Determination of fumonisin B1 and fumonisin B2 in processed maize containing foods for infants and young children. HPLC method with immunoaffinity column cleanup and fluorescence detection after pre-column derivatisation
Hardcopy , PDF
English
30-06-2015
Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Procedure
7 Calculation
8 Precision
9 Test report
Annex A (informative) - Typical chromatograms
Annex B (informative) - Precision data
Annex C (informative) - Comparison between the method
in this document and EN 14352:2004 and
EN 13585:2001 on fumonisins in maize
Bibliography
Describes a method for the determination of fumonisin B1 (FB1) and fumonisin B2 (FB2) in processed maize-containing foods for infants and young children by high performance liquid chromatography (HPLC) with immunoaffinity cleanup and fluorescence detection (FLD).
| Committee |
AW/275
|
| DevelopmentNote |
Supersedes DD CEN/TS 16187 & 12/30273167 DC. (06/2015)
|
| DocumentType |
Standard
|
| Pages |
24
|
| PublisherName |
British Standards Institution
|
| Status |
Current
|
| Supersedes | |
| UnderRevision |
This European Standard specifies a method for the determination of fumonisin B 1 (FB 1) and fumonisin B 2 (FB 2) in processed maize-containing foods for infants and young children by high performance liquid chromatography (HPLC) with immunoaffinity cleanup and fluorescence detection (FLD). This method has been validated in an interlaboratory study via the analysis of both naturally contaminated and spiked samples ranging from 112µg/kg to 458µg/kg for FB 1+FB 2, 89µg/kg to 384µg/kg for FB 1 and 22µg/kg to 74µg/kg for FB 2. For further information on the validation, see Clause8 and AnnexB.
| Standards | Relationship |
| EN 16187:2015 | Identical |
| EN 13585:2001 | Foodstuffs - Determination of fumonisins B1 and B2 in maize - HPLC method with solid phase extraction clean-up |
| EN 14352:2004 | Foodstuffs - Determination of fumonisin B1 and B2 in maize based foods - HPLC method with immunoaffinity column clean up |
| ISO 3696:1987 | Water for analytical laboratory use — Specification and test methods |
| EN ISO 3696:1995 | Water for analytical laboratory use - Specification and test methods (ISO 3696:1987) |
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