BS EN ISO 10705-2:2001
Current
The latest, up-to-date edition.
Water quality. Detection and enumeration of bacteriophages Enumeration of somatic coliphages
Hardcopy , PDF
English
19-11-2001
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1 Scope
2 Normative references
3 Terms and definitions
4 Safety precautions
5 Principle
6 Diluent, culture media and reagents
6.1 Basic materials
6.2 Diluent
7 Apparatus and glassware
8 Microbiological reference cultures
9 Sampling
10 Preparation of test material
10.1 Culturing and maintenance of host strains
10.2 Calibration of absorbance measurements for
counts of viable microorganisms
11 Procedure
11.1 Preparation of inoculum cultures
11.2 Standard procedure
11.3 Method for samples with high bacterial
background flora
11.4 Samples with low phage counts
11.5 Presence/absence test
11.6 Quality assurance
12 Expression of results
12.1 Plaque count procedures (11.2 to 11.4)
12.2 Presence/absence test (11.5)
13 Test report
Annex A (normative) Culture media, reagents and
diluent
Annex B (informative) General description of
somatic bacteriophages
Annex C (informative) Culturing of bacteriaophage
X174
Bibliography
Describes a method for the detection and enumeration of somatic coliphages by incubating the sample with an appropriate host strain. Applicable to all kinds of water, sediments and sludge extracts, where necessary after dilution.
| Committee |
EH/3/4
|
| DevelopmentNote |
Renumbers and supersedes BS 6068-4.13(2000). Supersedes 96/561512 DC. (12/2001) Reviewed and confirmed by BSI, July 2008. (06/2008)
|
| DocumentType |
Standard
|
| Pages |
26
|
| PublisherName |
British Standards Institution
|
| Status |
Current
|
| Supersedes |
| Standards | Relationship |
| NBN EN ISO 10705-2 : 2001 | Identical |
| ISO 10705-2:2000 | Identical |
| NS EN ISO 10705-2 : 1ED 2001 | Identical |
| UNE-EN ISO 10705-2:2002 | Identical |
| NF EN ISO 10705-2 : 2001 | Identical |
| I.S. EN ISO 10705-2:2002 | Identical |
| UNI EN ISO 10705-2 : 2001 | Identical |
| NEN EN ISO 10705-2 : 2001 | Identical |
| DIN EN ISO 10705-2:2002-01 | Identical |
| EN ISO 10705-2:2001 | Identical |
| SN EN ISO 10705-2 : 2001 | Identical |
| ISO 5667-2:1991 | Water quality Sampling Part 2: Guidance on sampling techniques |
| ISO 8199:2005 | Water quality General guidance on the enumeration of micro-organisms by culture |
| ISO 5667-3:2012 | Water quality Sampling Part 3: Preservation and handling of water samples |
| ISO 31-0:1992 | Quantities and units Part 0: General principles |
| ISO 5667-1:2006 | Water quality Sampling Part 1: Guidance on the design of sampling programmes and sampling techniques |
| ISO 3696:1987 | Water for analytical laboratory use — Specification and test methods |
| ISO/IEC Guide 2:2004 | Standardization and related activities — General vocabulary |
| ISO 6887:1983 | Microbiology General guidance for the preparation of dilutions for microbiological examination |
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