BS EN ISO 17099:2017

Foreword
Introduction
1 Scope
2 Terms and definitions
3 Micronucleus assay methodology used
   in this standard
4 Confidentiality of personal information
5 Laboratory safety requirements
6 Calibration source(s), calibration curve, and
   minimum resolvable dose
7 Responsibility of the customer
8 Responsibility of the CBMN laboratory
9 Accidental overexposure involving few individuals
10 Population triage
11 Quality assurance and quality control
Annex A (informative) - Sample data sheet for recording
        micronuclei in binucleated cells
Annex B (informative) - Automation of micronuclei scoring
Annex C (informative) - Instructions for customer (sample)
Annex D (informative) - Sample questionnaire
Annex E (informative) - Sample of report for single
        assessment
Annex F (informative) - Example group sample report
Bibliography

Describes the following: a) confidentiality of personal information for the customer and the laboratory; b) laboratory safety requirements; c) radiation sources, dose rates, and ranges used for establishing the calibration reference dose-effect curves allowing the dose estimation from CBMN assay yields and the minimum resolvable dose; d) performance of blood collection, culturing, harvesting, and sample preparation for CBMN assay scoring; e) scoring criteria; f) conversion of micronucleus frequency in binucleated cells into an estimate of absorbed dose; g) reporting of results; h) quality assurance and quality control; and i) informative annexes containing examples of a questionnaire, instructions for customers, a microscope scoring data sheet, a sample report and advice on strengths and limitations of current automated systems for automated micronucleus scoring.

This International Standard addresses the following: confidentiality of personal information for the customer and the laboratory; laboratory safety requirements; radiation sources, dose rates, and ranges used for establishing the calibration reference dose-effect curves allowing the dose estimation from CBMN assay yields and the minimum resolvable dose; performance of blood collection, culturing, harvesting, and sample preparation for CBMN assay scoring; scoring criteria; conversion of micronucleus frequency in binucleated cells into an estimate of absorbed dose; reporting of results; quality assurance and quality control; informative annexes containing examples of a questionnaire, instructions for customers, a microscope scoring data sheet, a sample report and advice on strengths and limitations of current automated systems for automated micronucleus scoring.