BS EN ISO 21872-1:2017
Withdrawn
A Withdrawn Standard is one, which is removed from sale, and its unique number can no longer be used. The Standard can be withdrawn and not replaced, or it can be withdrawn and replaced by a Standard with a different number.
Microbiology of the food chain. Horizontal method for the determination of Vibrio spp. Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus
Hardcopy , PDF
16-03-2023
English
22-09-2017
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Culture media and reagents
6 Equipment and consumables
7 Sampling
8 Preparation of the test sample
9 Procedure
10 Expression of results
11 Performance characteristics of the method
12 Test report
Annex A (normative) - Diagram of procedure
Annex B (normative) - Composition and preparation
of the culture media and reagents
Annex C (informative) - Conventional PCR for the
detection of Vibrio parahaemolyticus,
thermostable direct haemolysin (tdh) and
thermostable direct related haemolysin (trh)
genes, Vibrio cholerae and Vibrio vulnificus
Annex D (informative) - Real-time PCR for the
detection of Vibrio parahaemolyticus,
thermostable direct haemolysin gene (tdh)
and Vibrio vulnificus
Annex E (informative) - Results of an interlaboratory
study
Bibliography
Defines a horizontal method for the detection of enteropathogenic Vibrio spp., which causes human illness in or via the intestinal tract.
| Committee |
AW/9
|
| DevelopmentNote |
Supersedes DD ISO/TS 21872-1. (10/2017)
|
| DocumentType |
Standard
|
| Pages |
44
|
| PublisherName |
British Standards Institution
|
| Status |
Withdrawn
|
| Supersedes | |
| UnderRevision |
This document specifies a horizontal method for the detection of enteropathogenic Vibrio spp., which causes human illness in or via the intestinal tract. The species detectable by the methods specified include Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus. It is applicable to the following: products intended for human consumption and the feeding of animals; environmental samples in the area of food production and food handling. NOTE1 This method may not be appropriate in every detail for certain products (see Introduction). NOTE2 The World Health Organization (WHO) has identified that V. parahaemolyticus, V. cholerae and V. vulnificus are the major food-borne Vibrio spp. However, the method in this document can also be appropriate for the identification of other Vibrio spp. causing illness in humans. [1]
| Standards | Relationship |
| ISO 21872-1:2017 | Identical |
| EN ISO 21872-1:2017 | Identical |
| ISO 22174:2005 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions |
| ISO 5725-1:1994 | Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions |
| ISO 6887-1:2017 | Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial suspension and decimal dilutions |
| ISO 7218:2007 | Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations |
| ISO/TS 20836:2005 | Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Performance testing for thermal cyclers |
| ISO 20837:2006 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Requirements for sample preparation for qualitative detection |
| ISO 22118:2011 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens — Performance characteristics |
| ISO 20838:2006 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Requirements for amplification and detection for qualitative methods |
| ISO 11133:2014 | Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media |
| ISO 5725-2:1994 | Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method |
| ISO 6887-3:2017 | Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 3: Specific rules for the preparation of fish and fishery products |
| ISO 17468:2016 | Microbiology of the food chain — Technical requirements and guidance on establishment or revision of a standardized reference method |
| ISO 22119:2011 | Microbiology of food and animal feeding stuffs — Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions |
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