CEN ISO/TS 17919:2013
Current
The latest, up-to-date edition.
Microbiology of the food chain - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Detection of botulinum type A, B, E and F neurotoxin-producing clostridia (ISO/TS 17919:2013)
06-11-2013
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Symbols and abbreviated terms
5 Principle
6 Reagents
7 Apparatus and equipment
8 Sampling
9 Procedure
Annex A (normative) - Flow-diagram of the procedure
Annex B (informative) - Multiplex PCR assays for detection
of genes encoding botulinum neurotoxin types A, B, E,
and F using agarose gel electrophoresis
Annex C (informative) - Assays for detection of genes
encoding botulinum neurotoxin types A, B, E, and
F using real-time PCR
Annex D (informative) - Preparation of C. botulinum spores
Bibliography
ISO/TS 17919:2013 specifies a horizontal method for the molecular detection of clostridia carrying botulinum neurotoxin A, B, E, and F genes by a PCR method. This method detects the genes and not the toxins, therefore a positive result does not necessarily mean the presence of these toxins in the sample investigated. ISO/TS 17919:2013 is applicable to products for human consumption, animal feed, and environmental samples.The PCR assays for detection of genetic sequences encoding specific toxin types are described in annexes.
| Committee |
CEN/TC 463
|
| DocumentType |
Technical Specification
|
| PublisherName |
Comite Europeen de Normalisation
|
| Status |
Current
|
| Standards | Relationship |
| DIN CEN ISO/TS 17919;DIN SPEC 10793:2014-03 | Identical |
| UNI CEN ISO/TS 17919 : 2013 | Identical |
| XP CEN ISO/TS 17919 : 2013 XP | Identical |
| S.R. CEN ISO/TS 17919:2013 | Identical |
| NEN NPR CEN ISO/TS 17919 : 2013 | Identical |
| PD CEN ISO/TS 17919:2013 | Identical |
| UNE-CEN ISO/TS 17919:2013 | Identical |
| ISO/TS 17919:2013 | Identical |
| PNE-FprCEN ISO/TS 17919 | Identical |
| ISO 22174:2005 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions |
| ISO 6887-1:2017 | Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial suspension and decimal dilutions |
| ISO 7218:2007 | Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations |
| ISO 6887-6:2013 | Microbiology of food and animal feed — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 6: Specific rules for the preparation of samples taken at the primary production stage |
| ISO 20837:2006 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Requirements for sample preparation for qualitative detection |
| ISO 22118:2011 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens — Performance characteristics |
| ISO 6887-5:2010 | Microbiology of food and animal feeding stuffs Preparation of test samples, initial suspension and decimal dilutions for microbiological examination Part 5: Specific rules for the preparation of milk and milk products |
| ISO 20838:2006 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Requirements for amplification and detection for qualitative methods |
| ISO 6887-4:2017 | Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 4: Specific rules for the preparation of miscellaneous products |
| ISO 6887-3:2017 | Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 3: Specific rules for the preparation of fish and fishery products |
| ISO 6887-2:2017 | Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 2: Specific rules for the preparation of meat and meat products |
| ISO 22119:2011 | Microbiology of food and animal feeding stuffs — Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions |
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