CLSI M27 A3 : 3ED 2008

Abstract
Committee Membership
Foreword
Updated Information in This Edition
1 Scope
2 Introduction
3 Standard Precautions
4 Definitions
5 Indications for Performing Susceptibility Tests
6 Antifungal Agents
  6.1 Source
  6.2 Weighing Antifungal Powders
  6.3 Preparing Stock Solutions
  6.4 Number of Concentrations Tested
  6.5 Selection of Antifungal Agents for Routine Testing
       and Reporting
7 Test Procedures
  7.1 Broth Medium
  7.2 Preparing Diluted Antifungal Agents
  7.3 Inoculum Preparation
  7.4 Inoculating RPMI-1640 Medium
  7.5 Incubation
  7.6 Reading Results
  7.7 Interpretation of Results
  7.8 Broth Microdilution Modifications
  7.9 Trailing Growth and the Impact of Time of Reading
  7.10 Other Modifications
8 Quality Control
  8.1 Purpose
  8.2 QC Responsibilities
  8.3 Selecting Reference Strains
  8.4 Storing Reference Strains
  8.5 Routine Use of Reference Strains
  8.6 Batch of Medium and Lot of Plasticware Control
  8.7 QC Frequency
  8.8 Other Control Procedures
  8.9 QC Strains
References
Appendix A. RPMI 1640 Medium
Appendix B. McFarland 0.5 Barium Sulfate Turbidity Standard
Summary of Delegate Comments and Committee Responses
The Quality Management System Approach
Related CLSI Reference Materials

Describes the selection and preparation of antifungal agents; implementation and interpretation of test procedures; and quality control requirements for susceptibility testing of yeasts that cause invasive fungal infections.